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Current criteria: Na-phosphate buffer
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769 CLOSTRIDIUM VINCENTII MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Sea Salt | 11.988 | - |
| Lactose | 1.998 | 5.837 |
| NaHCO3 | 0.999 | 11.892 |
| Yeast extract | 0.999 | - |
| Trypticase peptone | 0.4 | - |
| L-Cysteine HCl x H2O | 0.3 | 1.706 |
| Na2S x 9 H2O | 0.3 | 1.248 |
| NH4NO3 | 0.1 | - |
| Sodium resazurin | 5.0e-4 | 0.002 |
| Pyridoxine hydrochloride | 1.0e-4 | 4.9e-4 |
| Nicotinic acid | 5.0e-5 | 4.1e-4 |
| p-Aminobenzoic acid | 5.0e-5 | 3.6e-4 |
| (DL)-alpha-Lipoic acid | 5.0e-5 | 2.4e-4 |
| Calcium D-(+)-pantothenate | 5.0e-5 | 2.1e-4 |
| Thiamine HCl | 5.0e-5 | 1.5e-4 |
| Riboflavin | 5.0e-5 | 1.3e-4 |
| Biotin | 2.0e-5 | 8.2e-5 |
| Folic acid | 2.0e-5 | 4.5e-5 |
| Vitamin B12 | 1.0e-6 | 7.4e-7 |
| Na-phosphate buffer | - | 4 |
| Distilled water | - | - |
Solutions:
- 1 ml Wolin's vitamin solution (10x)
Gas phase:
- 80 % N2
- 20 % CO2