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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na2HPO4 x 3 H2O

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511 METHANOPYRUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 4.469 21.981
NaHCO3 0.993 11.821
Na2SO4 0.804 5.663
Na2S x 9 H2O 0.497 2.067
KCl 0.298 3.996
NH4Cl 0.248 4.641
KH2PO4 0.089 0.657
K2HPO4 x 3 H2O 0.07 0.399
NaBr 0.04 0.386
MgSO4 x 7 H2O 0.03 0.121
SrCl2 x 6 H2O 0.018 0.067
Nitrilotriacetic acid 0.015 0.078
H3BO3 0.013 0.209
NaCl 0.01 0.17
MnSO4 x H2O 0.005 0.029
Na2WO4 x 2 H2O 0.002 0.006
(NH4)2Ni(SO4)2 0.002 0.005
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
Na-silicate 9.9e-4 0.008
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
NaF 6.0e-4 0.014
Sodium resazurin 5.0e-4 0.002
KNO3 4.0e-4 0.004
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
Pyridoxine hydrochloride 9.9e-5 4.8e-4
Na2MoO4 x 2 H2O 9.9e-5 4.1e-4
CuSO4 x 5 H2O 9.9e-5 4.0e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
KI 1.2e-5 4.8e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Na2HPO4 x 3 H2O 2.5e-6 1.7e-5
Vitamin B12 9.9e-7 7.3e-7
Distilled water - -
Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 10 ml Marine trace elements solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2