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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Na2HPO4 x 6 H2O
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1733 MEDIUM FOR HYDROGENOPHILUS
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 x 6 H2O | 3.277 | 23.083 |
| KH2PO4 | 2.599 | 19.097 |
| NH4Cl | 1.13 | 21.124 |
| NaHCO3 | 0.565 | 6.725 |
| CaCl2 x 2 H2O | 0.113 | 0.769 |
| Sodium lactate | 0.04 | 0.357 |
| MgSO4 x 7 H2O | 0.01 | 0.041 |
| Yeast extract | 0.002 | - |
| H3BO3 | 0.002 | 0.027 |
| CoCl2 x 6 H2O | 0.001 | 0.009 |
| ZnSO4 x 7 H2O | 5.6e-4 | 0.002 |
| Fe(NH4)citrate | 4.0e-4 | - |
| MnCl2 x 4 H2O | 1.7e-4 | 8.6e-4 |
| Na2MoO4 x 2 H2O | 1.7e-4 | 7.0e-4 |
| NiCl2 x 6 H2O | 1.1e-4 | 8.7e-4 |
| CuCl2 x 2 H2O | 5.6e-5 | 3.3e-4 |
| Distilled water | - | - |
Solutions:
- 5 ml Trace element solution SL 6
J774 MINERAL MEDIUM FOR HYDROGENOPHILUS ISLANDICUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Na2HPO4 x 6 H2O | 2.886 | 20.327 |
| KH2PO4 | 2.289 | 16.817 |
| NH4Cl | 0.995 | 18.602 |
| NaHCO3 | 0.498 | 5.922 |
| CaCl2 x 2 H2O | 0.1 | 0.677 |
| H3BO3 | 0.001 | 0.024 |
| CoCl2 x 6 H2O | 1.0e-3 | 0.008 |
| ZnSO4 x 7 H2O | 5.0e-4 | 0.002 |
| Na2MoO4 x 2 H2O | 1.5e-4 | 6.2e-4 |
| MnCl2 x 4 H2O | 1.5e-4 | - |
| NiCl2 x 6 H2O | 1.0e-4 | 7.7e-4 |
| CuCl2 x 2 H2O | 5.0e-5 | 2.9e-4 |
| Distilled water | - | - |
| MgSO4 x 7 H2O | - | - |
| Fe(NH4)citrate | - | - |
Solutions:
- 5 ml SL-6 trace element solution