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Search for media using compounds and concentrations.

The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na2S2O3 x 5 H2O

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28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Na2S2O3 x 5 H2O 0.5 2.015
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

35a THIOMONAS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		19.418 57.733
Na2S2O3 x 5 H2O 4.854 19.559
KH2PO4 2.913 21.403
Yeast extract 0.971 -
CaCl2 x 2 H2O 0.136 0.925
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Solutions:
  • 100 ml Solution B
  • 20 ml Solution C
  • 10 ml Solution D
  • 900 ml Soution A

36 THIOBACILLUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		12 35.679
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
CaCl2 0.1 0.901
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61 THERMOANAEROBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sucrose 10 29.214
Tryptone 10 -
Yeast extract 2 -
Na2SO3 0.2 1.587
FeSO4 x 7 H2O 0.2 0.719
Na2S2O3 x 5 H2O 0.08 0.322
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Gas phase:
  • 100 % N2

68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		15 44.599
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
MgSO4 x 7 H2O 0.8 3.246
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Solutions:
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

69 STARKEYA NOVELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.892 -
Na2HPO4 x 12 H2O 10.524 74.133
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
NH4Cl 0.298 5.568
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Solutions:
  • 900 ml Solution A
  • 50 ml Solution B
  • 5 ml Solution C
  • 50 ml Solution D
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

71 ACIDITHIOBACILLUS THIOOXIDANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 5 20.146
(NH4)2SO4 3 22.703
KH2PO4 3 22.045
MgSO4 x 7 H2O 0.5 2.029
CaCl2 x 2 H2O 0.25 1.701
Distilled water - -

88c ZESTOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 2 8.058
(NH4)2SO4 1.3 9.838
Tryptone peptone 1 -
Yeast extract 1 -
Na2S x 9 H2O 0.5 2.082
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Solutions:
  • 10 ml Allen's trace element solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104a ANAEROBACULUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Meat peptone 5 -
Na2S2O3 x 5 H2O 2.5 10.073
D-Glucose 1 5.551
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Solutions:
  • 40 ml Salt solution
Gas phase:
  • 100 % N2

113 THIOBACILLUS DENITRIFICANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2S2O3 x 5 H2O 4.985 20.086
KNO3 1.994 19.723
KH2PO4 1.994 14.653
Na2CO3 0.997 9.407
NH4Cl 0.997 18.639
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Solutions:
  • 962 ml Solution A
  • 20 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 2 ml Trace element solution SL-4
Gas phase:
  • 100 % N2
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