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Current criteria: Na2SeO3

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52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
D-(+)-biotin 2.0e-5 8.2e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

60 MOORELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 17.946 99.612
K2HPO4 6.979 40.069
Yeast extract 4.985 -
Tryptone 4.985 -
KH2PO4 4.487 32.969
Na2CO3 1.994 18.814
(NH4)2SO4 0.499 3.773
L-Cysteine HCl x H2O 0.299 1.703
Na2S x 9 H2O 0.299 1.245
MgSO4 x 7 H2O 0.1 0.405
Fe(NH4)2(SO4)2 0.04 -
Na2MoO4 x 2 H2O 0.002 0.01
Sodium resazurin 5.0e-4 0.002
Na2SeO3 x 5 H2O 1.5e-4 5.7e-4
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Solutions:
  • 843 ml Solution A
  • 50 ml Solution B
  • 50 ml Solution C
  • 40 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
Gas phase:
  • 100 % CO2

76 GOTTSCHALKIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Uric acid 2 11.897
Na2CO3 1.5 14.153
Yeast extract 1 -
K2HPO4 0.91 5.225
KOH 0.67 11.942
Na-thioglycolate 0.5 4.382
MgSO4 x 7 H2O 0.25 1.014
CaCl2 x 2 H2O 0.015 0.102
FeSO4 x 7 H2O 0.006 0.022
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 -
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
Gas phase:
  • 100 % N2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

124 DESULFOFARCIMEN MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.487 53.407
Na2SO4 2.832 19.935
Na-acetate 1.396 17.015
Na-butyrate 1.396 12.679
NaCl 1.167 19.961
Yeast extract 0.997 -
MgCl2 x 6 H2O 0.399 1.962
Na2S x 9 H2O 0.399 1.66
KCl 0.299 4.012
NH4Cl 0.269 5.033
KH2PO4 0.199 1.465
CaCl2 x 2 H2O 0.15 1.017
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
ZnCl2 7.0e-5 5.1e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
Biotin 2.0e-5 8.0e-5
Folic acid 2.0e-5 4.4e-5
Na2WO4 x 2 H2O 7.8e-6 2.4e-5
Na2SeO3 x 5 H2O 5.9e-6 2.2e-5
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
Biotin 2.0e-5 8.0e-5
Folic acid 2.0e-5 4.4e-5
Na2WO4 x 2 H2O 7.8e-6 2.4e-5
Na2SeO3 x 5 H2O 5.9e-6 2.2e-5
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135b ACETOBACTERIUM DEHALOGENANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
K2HPO4 0.447 2.568
KH2PO4 0.328 2.41
MgSO4 x 7 H2O 0.06 0.242
Nitrilotriacetic acid 0.03 0.156
NaCl 0.02 0.34
MnSO4 x H2O 0.01 0.059
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.014
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 6.0e-4 0.005
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 4.0e-4 8.4e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.2e-4
CuSO4 x 5 H2O 2.0e-4 8.0e-4
Pyridoxine hydrochloride 9.9e-5 4.8e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
Na2WO4 x 2 H2O 8.0e-6 2.4e-5
Na2SeO3 x 5 H2O 6.0e-6 2.3e-5
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 15 ml Na-syringate solution (0.3 M)
Gas phase:
  • 80 % N2
  • 20 % CO2

135c MOORELLA THERMOACETICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Fructose
optional 		9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
NaHCO3 0.979 11.659
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
Riboflavin 4.9e-5 1.3e-4
Biotin 2.0e-5 8.0e-5
Folic acid 2.0e-5 4.4e-5
Na2WO4 x 2 H2O 7.8e-6 2.4e-5
Na2SeO3 x 5 H2O 5.9e-6 2.2e-5
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

141 METHANOGENIUM MEDIUM (H2/CO2)

Compound Conc. [g/L] Conc. [mM]
NaCl 17.769 304.055
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
MgSO4 x 7 H2O 3.406 13.818
Trypticase peptone 1.974 -
Yeast extract 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
CaCl2 x 2 H2O 0.138 0.94
K2HPO4 0.138 0.793
Nitrilotriacetic acid 0.015 0.077
MnSO4 x H2O 0.005 0.029
Fe(NH4)2(SO4)2 x 6 H2O 0.002 -
CoSO4 x 7 H2O 0.002 0.011
ZnSO4 x 7 H2O 0.002 0.006
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 4.9e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 9.9e-5 0.002
Na2MoO4 x 2 H2O 9.9e-5 4.1e-4
CuSO4 x 5 H2O 9.9e-5 4.0e-4
Pyridoxine hydrochloride 9.9e-6 4.8e-5
Nicotinic acid 4.9e-6 4.0e-5
p-Aminobenzoic acid 4.9e-6 3.6e-5
(DL)-alpha-Lipoic acid 4.9e-6 2.4e-5
Calcium D-(+)-pantothenate 4.9e-6 2.1e-5
Thiamine HCl 4.9e-6 1.5e-5
Riboflavin 4.9e-6 1.3e-5
Na2WO4 x 2 H2O 3.9e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
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Solutions:
  • 10 ml Wolin's vitamin solution
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % H2
  • 20 % CO2
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