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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Na2SeO4

Your search received 78 results.

322 METHANOSPHAERA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.704 240.443
NaHCO3 3.891 46.318
KH2PO4 2.724 20.015
Trypticase peptone 1.946 -
Yeast extract 1.946 -
NH4Cl 0.973 18.186
NaCl 0.584 9.987
K2HPO4 0.584 3.351
Na-formate 0.486 7.152
Na-acetate 0.486 5.929
DL-Dithiothreitol 0.486 3.152
(NH4)2SO4 0.292 2.208
MgSO4 x 7 H2O 0.146 0.592
CaCl2 x 2 H2O 0.078 0.529
Nitrilotriacetic acid 0.015 0.076
MnSO4 x H2O 0.005 0.029
FeSO4 x 7 H2O 0.003 0.01
Na2SeO4 0.002 0.01
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Solutions:
  • 100 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 2 ml Wolin's vitamin solution (10x)
  • 3 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

534 MODIFIED SME MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 5.5 27.053
NaHCO3 2 23.808
Na2S2O3 x 5 H2O 2 8.058
KCl 0.65 8.719
NH4Cl 0.15 2.804
K2HPO4 0.15 0.861
NaBr 0.1 0.972
MgSO4 x 7 H2O 0.03 0.122
(NH4)2Ni(SO4)2 0.02 0.051
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Na2SeO4 1.0e-4 5.3e-4
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Solutions:
  • 10 ml Trace element solution
  • 30 ml Solution A
  • 30 ml Solution B
  • 1000 ml Modified Wolin's mineral solution II
Gas phase:
  • 80 % H2
  • 20 % CO2
  • 0.5 % O2

766 THERMOCOCCUS CHITINOPHAGUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 11.383 194.78
MgCl2 x 6 H2O 5.16 25.383
Na2SO4 1.901 13.385
CaCl2 0.534 4.807
(NH4)2SO4 0.5 3.784
KH2PO4 0.5 3.674
Na2S x 9 H2O 0.5 2.082
KCl 0.322 4.32
HCl 0.296 8.118
NaHCO3 0.093 1.108
Chitin 0.067 -
KBr 0.047 0.391
MgSO4 x 7 H2O 0.045 0.183
Nitrilotriacetic acid 0.023 0.118
H3BO3 0.013 0.204
SrCl2 0.012 0.073
MnSO4 x H2O 0.008 0.044
CoSO4 x 7 H2O 0.003 0.017
ZnSO4 x 7 H2O 0.003 0.009
CaCl2 x 2 H2O 0.002 0.01
FeSO4 x 7 H2O 0.002 0.005
NaF 0.001 0.035
Resazurin 1.0e-3 0.004
NiCl2 x 6 H2O 4.5e-4 0.003
(NH4)2Ni(SO4)2 3.0e-4 7.6e-4
AlK(SO4)2 x 12 H2O 3.0e-4 6.3e-4
Na2SeO4 1.5e-4 7.9e-4
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Solutions:
  • 4 ml Chitin stock solution
  • 15 ml Modified Wolin's mineral solution II
  • 485 ml Artifical sea water

792 PYROLOBUS FUMARII MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.723 13.393
KNO3 0.99 9.793
KH2PO4 0.495 3.638
KCl 0.327 4.383
Na2S x 9 H2O 0.149 0.618
NaBr 0.05 0.481
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.169
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 9.9e-5 0.002
Na2SeO4 9.9e-5 5.2e-4
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 1 ml Solution C
  • 10 ml Solution D
  • 1 ml Wolfe's mineral elixir
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % H2
  • 20 % CO2

792a THERMOVIBRIO AMMONIFICANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.725 13.406
KNO3 0.991 9.803
KH2PO4 0.496 3.641
KCl 0.327 4.387
Na2S x 9 H2O 0.149 0.619
NaBr 0.05 0.482
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 9.9e-5 0.002
Na2SeO4 9.9e-5 5.2e-4
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 1 ml Wolfe's mineral elixir
Gas phase:
  • 80 % H2
  • 20 % CO2

792b CAMINIBACTER MEDIATLANTICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.725 13.406
KNO3 0.991 9.803
KH2PO4 0.496 3.641
KCl 0.327 4.387
Na2S x 9 H2O 0.149 0.619
NaBr 0.05 0.482
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.9e-4 0.007
FeSO4 x 7 H2O 9.9e-4 0.004
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 9.9e-5 0.002
Na2SeO4 9.9e-5 5.2e-4
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Solutions:
  • 989 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 1 ml Wolfe's mineral elixir
Gas phase:
  • 80 % H2
  • 20 % CO2

792c NAUTILIA NITRATIREDUCENS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 2.739 13.473
KNO3 0.996 9.851
Na2CO3 0.996 9.397
KH2PO4 0.498 3.66
Na2S x 9 H2O 0.448 1.866
KCl 0.329 4.409
NaBr 0.05 0.484
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.17
SrCl2 x 6 H2O 0.007 0.026
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 1.0e-4 0.002
Na2SeO4 1.0e-4 5.3e-4
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Solutions:
  • 958 ml Solution A
  • 10 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 15 ml Solution E
  • 1 ml Wolfe's mineral elixir
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

1028 THERMINCOLA MEDIUM (CO)

Compound Conc. [g/L] Conc. [mM]
NH4Cl 0.997 18.639
Na2S x 9 H2O 0.997 4.151
NaHCO3 0.499 5.934
Na2CO3 0.499 4.703
KH2PO4 0.499 3.663
KCl 0.329 4.413
MgCl2 x 6 H2O 0.329 1.618
Na-acetate 0.199 2.431
Yeast extract 0.199 -
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 2.0e-4 9.7e-4
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 1.0e-4 0.002
Nicotinic acid 1.0e-4 8.1e-4
p-Aminobenzoic acid 1.0e-4 7.3e-4
Na2SeO4 1.0e-4 5.3e-4
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 2 ml Wolin's vitamin solution (10x)
Gas phase:
  • 90 % CO
  • 10 % N2

1030 SMITHELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.957 -
Trypticase peptone 1.957 -
Crotonic acid 1.683 19.549
NH4Cl 0.978 18.293
Na2CO3 0.978 9.232
MgCl2 x 6 H2O 0.978 4.813
2-Mercaptoethanesulfonic acid 0.489 3.44
KH2PO4 0.391 2.876
Na2S x 9 H2O 0.294 1.222
MgSO4 x 7 H2O 0.029 0.119
NaCl 0.01 0.167
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 9.8e-4 0.007
FeSO4 x 7 H2O 9.8e-4 0.004
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 1.8e-4 3.7e-4
H3BO3 9.8e-5 0.002
Na2SeO4 9.8e-5 5.2e-4
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 20 ml Na-crotonate solution (1 M)
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

1040 MARIDESULFOVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sea Salt 34.93 -
Na-DL-lactate 2.495 22.265
Na2CO3 0.998 9.416
Yeast extract 0.998 -
Na2S x 9 H2O 0.299 1.247
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 1.0e-4 0.002
Na2SeO4 1.0e-4 5.3e-4
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Solutions:
  • 1 ml Wolfe's mineral elixir
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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