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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: NaOH

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52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

76 GOTTSCHALKIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Uric acid 2 11.897
Na2CO3 1.5 14.153
Yeast extract 1 -
K2HPO4 0.91 5.225
KOH 0.67 11.942
Na-thioglycolate 0.5 4.382
MgSO4 x 7 H2O 0.25 1.014
CaCl2 x 2 H2O 0.015 0.102
FeSO4 x 7 H2O 0.006 0.022
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
Gas phase:
  • 100 % N2

78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
Resazurin 9.5e-4 0.004
p-Aminobenzoic acid 2.4e-4 0.002
alpha-lipoic acid 2.4e-4 0.001
Pantothenic acid 2.4e-4 0.001
Thiamine-HCl x 2 H2O 2.4e-4 6.3e-4
Riboflavin 2.4e-4 6.3e-4
Vitamin B12 2.4e-4 1.7e-4
Nicotine amide 1.2e-4 9.7e-4
Nicotinic acid 1.2e-4 9.6e-4
Biotin 9.5e-5 3.9e-4
Folic acid 9.5e-5 2.1e-4
Pyridoxamine hydrochloride 4.7e-5 2.3e-4
NaOH - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
Riboflavin 5.9e-5 1.6e-4
Calcium D-(+)-pantothenate 5.9e-5 -
Biotin 2.3e-5 9.6e-5
Folic acid 2.3e-5 5.3e-5
Vitamin B12 1.2e-6 8.6e-7
NaOH - -
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
Thiamine-HCl x 2 H2O 2.5e-4 6.7e-4
Riboflavin 2.5e-4 6.6e-4
Vitamin B12 2.5e-4 1.8e-4
Nicotine amide 1.3e-4 0.001
Nicotinic acid 1.3e-4 0.001
Biotin 1.0e-4 4.1e-4
Folic acid 1.0e-4 2.3e-4
Pyridoxamine hydrochloride 5.0e-5 2.4e-4
Calf serum - -
Vitamin K1 - -
KCl - -
Water - -
NaOH - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
Ethanol 0.004 0.082
Resazurin 1.0e-3 0.004
Tween 80 - -
Vitamin K1 - -
NaOH - -
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
Maltose 1 2.921
Cellobiose 1 2.921
Starch 1 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Sodium resazurin 5.0e-4 -
Water - -
Vitamin K1 - -
NaOH - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

110a CMC MEDIUM (N2/CO2)

Compound Conc. [g/L] Conc. [mM]
Ground beef 495.049 -
Casitone 29.703 -
K2HPO4 4.951 28.422
Yeast extract 4.951 -
D-Glucose 3.96 21.983
Na2CO3 1.485 14.012
D-Maltose 0.99 2.893
Cellobiose 0.99 2.892
Starch 0.99 -
L-Cysteine HCl x H2O 0.495 2.819
Sodium resazurin 5.0e-4 0.002
NaOH - -
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Solutions:
  • 1000 ml Meat filtrate
  • 10 ml Sugar mix
Gas phase:
  • 80 % N2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

124 DESULFOFARCIMEN MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.487 53.407
Na2SO4 2.832 19.935
Na-acetate 1.396 17.015
Na-butyrate 1.396 12.679
NaCl 1.167 19.961
Yeast extract 0.997 -
MgCl2 x 6 H2O 0.399 1.962
Na2S x 9 H2O 0.399 1.66
KCl 0.299 4.012
NH4Cl 0.269 5.033
KH2PO4 0.199 1.465
CaCl2 x 2 H2O 0.15 1.017
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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