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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Nitrilotriacetic acid

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86 CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 1 14.069
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
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86a MODIFIED CASTENHOLZ MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 25 74.332
Peptone 3 -
Monosodium glutamate 1 5.913
Yeast extract 1 -
NaNO3 0.689 8.106
Na2HPO4 x 2 H2O 0.14 0.986
KNO3 0.103 1.019
Nitrilotriacetic acid 0.1 0.523
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135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135b ACETOBACTERIUM DEHALOGENANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 1.988 -
Na2CO3 1.491 14.068
NH4Cl 0.994 18.584
Syringic acid 0.895 -
L-Cysteine HCl x H2O 0.497 2.83
K2HPO4 0.447 2.568
KH2PO4 0.328 2.41
MgSO4 x 7 H2O 0.06 0.242
Nitrilotriacetic acid 0.03 0.156
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 15 ml Na-syringate solution (0.3 M)
Gas phase:
  • 80 % N2
  • 20 % CO2

135c MOORELLA THERMOACETICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Fructose
optional 		9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
NaHCO3 0.979 11.659
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

141 METHANOGENIUM MEDIUM (H2/CO2)

Compound Conc. [g/L] Conc. [mM]
NaCl 17.769 304.055
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
MgSO4 x 7 H2O 3.406 13.818
Trypticase peptone 1.974 -
Yeast extract 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
CaCl2 x 2 H2O 0.138 0.94
K2HPO4 0.138 0.793
Nitrilotriacetic acid 0.015 0.077
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Solutions:
  • 10 ml Wolin's vitamin solution
  • 10 ml Modified Wolin's mineral solution
Gas phase:
  • 80 % H2
  • 20 % CO2

141a METHANOCALDOCOCCUS LAUENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.949 19.423
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

141b METHANOCULLEUS SP. MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.936 58.755
MgCl2 x 6 H2O 3.949 19.423
Yeast extract 1.974 -
Trypticase peptone 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

141c METHANOCOCCOIDES MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.936 58.755
Trimethylammonium chloride 4.936 51.646
MgCl2 x 6 H2O 3.949 19.423
Yeast extract 1.974 -
Trypticase peptone 1.974 -
Na-acetate 0.987 12.034
L-Cysteine HCl x H2O 0.494 2.81
Na2S x 9 H2O 0.494 2.055
KCl 0.336 4.502
NH4Cl 0.247 4.614
K2HPO4 0.138 0.793
MgSO4 x 7 H2O 0.03 0.12
Nitrilotriacetic acid 0.015 0.077
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Solutions:
  • 10 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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