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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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911 MIDDLEBROCK 7H11 AGAR

Compound Conc. [g/L] Conc. [mM]
Bovine albumin 5 -
Dextrose 2 10.092
NaCl 0.85 14.545
Agar 0.35 1.041
Oleic acid 0.05 0.177
KH2PO4 0.035 0.257
Na2HPO4 0.035 0.247
Pancreatic digest of casein 0.023 -
(NH4)2SO4 0.012 0.088
L-Glutamic acid 0.012 0.079
Sodium citrate 0.009 0.036
Catalase 0.004 -
MgSO4 0.001 0.01
Ferric ammonium citrate 9.3e-4 -
Pyridoxine 2.3e-5 1.4e-4
Malachite green 2.3e-5 6.4e-5
Biotin 1.2e-5 4.8e-5
Glycerol - -
Distilled water - -
Solutions:
  • 21 ml Bacto Middelbrook 7H11 agar (Difco 283810)
  • 100 ml Bacto Middelbrook OADC enrichment (Difco 212240):