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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

	Compound	Min. conc.	Max. conc.	Unit

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Current criteria: PIPES

Your search received 29 results.

Compound	Conc. [g/L]	Conc. [mM]
PIPES	14.896	49.263
Na-laurate	2.761	12.419
CaCl₂ x 2 H₂O	1.827	12.429
Na₂CO₃	1.49	14.054
Trypticase peptone	0.993	-
KH₂PO₄	0.497	3.649
NH₄Cl	0.397	7.426
NaCl	0.397	6.797
MgCl₂ x 6 H₂O	0.328	1.612
L-Cysteine HCl x H₂O	0.298	1.696
Na₂S x 9 H₂O	0.298	1.24
HCl	0.002	0.068
FeCl₂ x 4 H₂O	0.001	0.007
Sodium resazurin	5.0e-4	0.002
Pyridoxine hydrochloride	3.0e-4	0.001
Nicotinic acid	2.0e-4	0.002
Thiamine-HCl x 2 H₂O	2.0e-4	5.3e-4
CoCl₂ x 6 H₂O	1.9e-4	0.001
MnCl₂ x 4 H₂O	9.9e-5	5.0e-4
Calcium pantothenate	9.9e-5	2.1e-4
Vitamin B₁₂	9.9e-5	7.3e-5
p-Aminobenzoic acid	7.9e-5	5.8e-4
ZnCl₂	7.0e-5	5.1e-4
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
D-(+)-biotin	2.0e-5	8.1e-5
H₃BO₃	6.0e-6	9.6e-5
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Distilled water	-	-
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Solutions:

921 ml Solution A
30 ml Solution B
1 ml Solution C
25 ml Solution D
10 ml Solution E
10 ml Solution F
10 ml Solution G
1 ml Trace element solution SL-10
50 ml Clarified rumen fluid
1 ml Seven vitamins solution

Gas phase:

80 % N2
20 % CO2

Compound	Conc. [g/L]	Conc. [mM]
PIPES	14.896	49.263
Na₂SO₄	2.781	19.576
Na-laurate	2.761	12.419
CaCl₂ x 2 H₂O	1.827	12.429
Na₂CO₃	1.49	14.054
KH₂PO₄	0.497	3.649
NH₄Cl	0.397	7.426
NaCl	0.397	6.797
MgCl₂ x 6 H₂O	0.328	1.612
L-Cysteine HCl x H₂O	0.298	1.696
Na₂S x 9 H₂O	0.298	1.24
HCl	0.002	0.068
FeCl₂ x 4 H₂O	0.001	0.007
Sodium resazurin	5.0e-4	0.002
Pyridoxine hydrochloride	3.0e-4	0.001
Nicotinic acid	2.0e-4	0.002
Thiamine-HCl x 2 H₂O	2.0e-4	5.3e-4
CoCl₂ x 6 H₂O	1.9e-4	0.001
MnCl₂ x 4 H₂O	9.9e-5	5.0e-4
Calcium pantothenate	9.9e-5	2.1e-4
Vitamin B₁₂	9.9e-5	7.3e-5
p-Aminobenzoic acid	7.9e-5	5.8e-4
ZnCl₂	7.0e-5	5.1e-4
Na₂MoO₄ x 2 H₂O	3.6e-5	1.5e-4
NiCl₂ x 6 H₂O	2.4e-5	1.8e-4
D-(+)-biotin	2.0e-5	8.1e-5
H₃BO₃	6.0e-6	9.6e-5
CuCl₂ x 2 H₂O	2.0e-6	1.2e-5
Distilled water	-	-
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Solutions:

921 ml Solution A
30 ml Solution B
1 ml Solution C
25 ml Solution D
10 ml Solution E
10 ml Solution F
10 ml Solution G
1 ml Trace element solution SL-10
1 ml Seven vitamins solution

Gas phase:

80 % N2
20 % CO2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	87.8	1502.4
MgCl₂ x 6 H₂O	20.3	99.851
PIPES	12.096	40.004
CaCl₂ x 2 H₂O	7.35	49.995
Glucose	5	27.753
Yeast extract	5	-
KCl	3.7	49.631
L-Cysteine HCl x H₂O	0.5	2.847
Resazurin	1.0e-4	4.4e-4
Distilled water	-	-
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Compound	Conc. [g/L]	Conc. [mM]
PIPES	3.363	11.122
KCl	1.978	26.536
Peptone	0.989	-
Na₂S x 9 H₂O	0.495	2.059
Yeast extract	0.495	-
NH₄Cl	0.099	1.849
MgSO₄ x 7 H₂O	0.03	0.12
K₂HPO₄	0.02	0.114
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.169
MnSO₄ x H₂O	0.005	0.029
CoSO₄ x 7 H₂O	0.002	0.011
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	9.9e-4	0.007
FeSO₄ x 7 H₂O	9.9e-4	0.004
Sodium resazurin	4.9e-4	0.002
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	9.9e-5	0.002
Pyridoxine hydrochloride	9.9e-5	4.8e-4
Na₂MoO₄ x 2 H₂O	9.9e-5	4.1e-4
CuSO₄ x 5 H₂O	9.9e-5	4.0e-4
Nicotinic acid	4.9e-5	4.0e-4
p-Aminobenzoic acid	4.9e-5	3.6e-4
(DL)-alpha-Lipoic acid	4.9e-5	2.4e-4
Calcium D-(+)-pantothenate	4.9e-5	2.1e-4
Thiamine HCl	4.9e-5	1.5e-4
Riboflavin	4.9e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Vitamin B₁₂	9.9e-7	7.3e-7
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
Sea Salt	30	-
PIPES	6	19.843
D-Glucose	2.5	13.877
Tryptone	1	14.069
Yeast extract	1	-
L-Cysteine HCl x H₂O	0.5	2.847
Na₂S x 9 H₂O	0.5	2.082
Sodium resazurin	5.0e-4	-
Distilled water	-	-
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Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	15	256.674
Maltose	3.5	10.225
PIPES	3.4	11.245
Na-acetate x 3 H₂O	2.72	-
Trypticase peptone	2	-
Yeast extract	2	-
NH₄Cl	1	18.695
MgCl₂ x 6 H₂O	0.5	2.459
Na₂S x 9 H₂O	0.5	2.082
KH₂PO₄	0.35	2.572
K₂HPO₄	0.35	2.009
KCl	0.2	2.683
CaCl₂ x 2 H₂O	0.1	0.68
Sodium resazurin	5.0e-4	-
Distilled water	-	-
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Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
NaCl	26	444.901
Maltose	3.5	10.225
PIPES	3.4	11.245
Na-acetate x 3 H₂O	2.72	-
Trypticase peptone	2	-
Yeast extract	2	-
NH₄Cl	1	18.695
MgCl₂ x 6 H₂O	0.5	2.459
Na₂S x 9 H₂O	0.5	2.082
KH₂PO₄	0.35	2.572
K₂HPO₄	0.35	2.009
KCl	0.2	2.683
CaCl₂ x 2 H₂O	0.1	0.68
Sodium resazurin	5.0e-4	-
Distilled water	-	-
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Gas phase:

100 % N2

Compound	Conc. [g/L]	Conc. [mM]
PIPES	3.6	11.906
Tryptone	1	14.069
Sucrose	1	2.921
Yeast extract	0.5	-
NH₄Cl	0.33	6.169
KCl	0.33	4.427
KNO₃	0.33	3.264
MgCl₂ x 6 H₂O	0.33	1.623
MgSO₄ x 7 H₂O	0.003	0.012
Nitrilotriacetic acid	0.002	0.008
NaCl	1.0e-3	0.017
MnSO₄ x H₂O	5.0e-4	0.003
CoSO₄ x 7 H₂O	1.8e-4	0.001
ZnSO₄ x 7 H₂O	1.8e-4	6.3e-4
CaCl₂ x 2 H₂O	1.0e-4	6.8e-4
FeSO₄ x 7 H₂O	1.0e-4	3.6e-4
NiCl₂ x 6 H₂O	3.0e-5	2.3e-4
AlK(SO₄)₂ x 12 H₂O	2.0e-5	4.2e-5
H₃BO₃	1.0e-5	1.6e-4
Pyridoxine hydrochloride	1.0e-5	4.9e-5
Na₂MoO₄ x 2 H₂O	1.0e-5	4.1e-5
CuSO₄ x 5 H₂O	1.0e-5	4.0e-5
Nicotinic acid	5.0e-6	4.1e-5
p-Aminobenzoic acid	5.0e-6	3.6e-5
(DL)-alpha-Lipoic acid	5.0e-6	2.4e-5
Thiamine HCl	5.0e-6	1.5e-5
Riboflavin	5.0e-6	1.3e-5
Calcium D-(+)-pantothenate	5.0e-6	-
Biotin	2.0e-6	8.2e-6
Folic acid	2.0e-6	4.5e-6
Na₂WO₄ x 2 H₂O	4.0e-7	1.2e-6
Na₂SeO₃ x 5 H₂O	3.0e-7	1.1e-6
Vitamin B₁₂	1.0e-7	7.4e-8
Distilled water	-	-
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Solutions:

10 ml Trace element solution
30 ml Solution A
30 ml Solution B
1000 ml Modified Wolin's mineral solution II
1 ml Wolin's vitamin solution
1 ml Modified Wolin's mineral solution II

Compound	Conc. [g/L]	Conc. [mM]
Sea Salt	35	-
Elemental sulphur	5	-
Peptone	4	-
PIPES	3.46	11.443
Yeast extract	1	-
NH₄Cl	0.5	9.348
KH₂PO₄	0.35	2.572
Na₂S x 9 H₂O	0.25	1.041
CaCl₂	0.2	1.802
FeCl₃	0.007	0.041
Na₂WO₄	0.003	0.01
Resazurin	1.0e-4	4.4e-4
Distilled water	-	-
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Compound	Conc. [g/L]	Conc. [mM]
PIPES	6.627	21.917
Maltose	4.946	14.448
Yeast extract	4.946	-
MgCl₂ x 2 H₂O	0.89	6.783
L-Cysteine HCl x H₂O	0.495	2.816
KH₂PO₄	0.445	3.271
KCl	0.326	4.378
NH₄Cl	0.247	4.623
MgSO₄ x 7 H₂O	0.03	0.12
Nitrilotriacetic acid	0.015	0.078
NaCl	0.01	0.169
MnSO₄ x H₂O	0.005	0.029
CoSO₄ x 7 H₂O	0.002	0.011
ZnSO₄ x 7 H₂O	0.002	0.006
CaCl₂ x 2 H₂O	9.9e-4	0.007
FeSO₄ x 7 H₂O	9.9e-4	0.004
Sodium resazurin	4.9e-4	0.002
NiCl₂ x 6 H₂O	3.0e-4	0.002
AlK(SO₄)₂ x 12 H₂O	2.0e-4	4.2e-4
H₃BO₃	9.9e-5	0.002
Pyridoxine hydrochloride	9.9e-5	4.8e-4
Na₂MoO₄ x 2 H₂O	9.9e-5	4.1e-4
CuSO₄ x 5 H₂O	9.9e-5	4.0e-4
Nicotinic acid	4.9e-5	4.0e-4
p-Aminobenzoic acid	4.9e-5	3.6e-4
(DL)-alpha-Lipoic acid	4.9e-5	2.4e-4
Calcium D-(+)-pantothenate	4.9e-5	2.1e-4
Thiamine HCl	4.9e-5	1.5e-4
Riboflavin	4.9e-5	1.3e-4
Biotin	2.0e-5	8.1e-5
Folic acid	2.0e-5	4.5e-5
Na₂WO₄ x 2 H₂O	4.0e-6	1.2e-5
Na₂SeO₃ x 5 H₂O	3.0e-6	1.1e-5
Vitamin B₁₂	9.9e-7	7.3e-7
Distilled water	-	-
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Solutions:

10 ml Modified Wolin's mineral solution
1 ml Wolin's vitamin solution (10x)

Gas phase:

100 % N2

Show 1 to 10 (29 total)

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