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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Pantothenic acid

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78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
Resazurin 9.5e-4 0.004
p-Aminobenzoic acid 2.4e-4 0.002
alpha-lipoic acid 2.4e-4 0.001
Pantothenic acid 2.4e-4 0.001
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

461 MINERAL MEDIUM (NAGEL AND ANDREESEN)

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.441 10.153
MgSO4 x 7 H2O 0.497 2.017
NH4Cl 0.298 5.575
KH2PO4 0.249 1.826
NaCl 0.05 0.85
CaCl2 0.01 0.09
MnSO4 0.01 0.066
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.007
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
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Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10

461a MINERAL MEDIUM WITH QUINOLINE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.31 9.23
MgSO4 x 7 H2O 0.452 1.833
Quinoline 0.273 2.112
NH4Cl 0.271 5.068
KH2PO4 0.226 1.66
NaCl 0.045 0.773
CaCl2 0.009 0.081
MnSO4 0.009 0.06
HCl 0.002 0.062
FeCl2 x 4 H2O 0.001 0.007
p-Aminobenzoic acid 2.3e-4 0.002
alpha-lipoic acid 2.3e-4 0.001
Pantothenic acid 2.3e-4 0.001
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Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10
  • 100 ml Quinoline solution

461b MINERAL MEDIUM WITH PYRROLIC ACID

Compound Conc. [g/L] Conc. [mM]
Pyrrole-2-carboxylic acid 11 99.01
Na2HPO4 x 2 H2O 1.427 10.053
MgSO4 x 7 H2O 0.492 1.997
NH4Cl 0.295 5.52
KH2PO4 0.246 1.808
NaCl 0.049 0.842
CaCl2 0.01 0.089
MnSO4 0.01 0.065
HCl 0.002 0.067
FeCl2 x 4 H2O 0.001 0.007
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
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Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10
  • 10 ml Pyrrole-2-carboxylic acid solution

461c MINERAL MEDIUM WITH O-NITROPHENOL

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.153 8.123
MgSO4 x 7 H2O 0.398 1.613
NH4Cl 0.239 4.46
KH2PO4 0.199 1.461
2-Nitrophenol 0.1 0.719
NaCl 0.04 0.68
CaCl2 0.008 0.072
MnSO4 0.008 0.053
HCl 0.002 0.055
FeCl2 x 4 H2O 0.001 0.006
p-Aminobenzoic acid 2.0e-4 0.001
alpha-lipoic acid 2.0e-4 9.6e-4
Pantothenic acid 2.0e-4 9.1e-4
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Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10
  • 200 ml Nitrophenol solution

463 NITRILOTRIACETATE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Nitrilotriacetate 1 5.232
MgSO4 x 7 H2O 1 4.057
Na2HPO4 x 2 H2O 0.41 2.888
KH2PO4 0.26 1.911
CaCl2 x 2 H2O 0.2 1.36
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.2e-4 9.2e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 6.8e-5 5.0e-4
H3BO3 6.2e-5 0.001
p-Aminobenzoic acid 5.0e-5 3.6e-4
alpha-lipoic acid 5.0e-5 2.4e-4
Pantothenic acid 5.0e-5 2.3e-4
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin solution

463a EDTA-MEDIUM

Compound Conc. [g/L] Conc. [mM]
MgSO4 x 7 H2O 1 4.057
EDTA 0.5 1.711
Na2HPO4 x 2 H2O 0.41 2.888
CaCl2 x 2 H2O 0.4 2.721
KH2PO4 0.26 1.911
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.2e-4 9.2e-4
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 6.8e-5 5.0e-4
H3BO3 6.2e-5 0.001
p-Aminobenzoic acid 5.0e-5 3.6e-4
alpha-lipoic acid 5.0e-5 2.4e-4
Pantothenic acid 5.0e-5 2.3e-4
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin solution

465g MINERAL MEDIUM WITH CYANURIC ACID AS NITROGEN SOURCE

Compound Conc. [g/L] Conc. [mM]
Glycerol 5.5 59.724
Na2HPO4 x 2 H2O 3.465 24.411
KH2PO4 0.99 7.276
Cyanuric acid 0.645 4.997
MgCl2 x 6 H2O 0.099 0.487
p-Aminobenzoic acid 5.0e-4 0.004
alpha-lipoic acid 5.0e-4 0.002
Pantothenic acid 5.0e-4 0.002
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Solutions:
  • 1 ml Trace element solution SL-4
  • 10 ml Vitamin solution

465h MINERALMEDIUM WITH 6-METHYLNICOTINATE

Compound Conc. [g/L] Conc. [mM]
6-methylnicotinate 5 -
Na2HPO4 x 2 H2O 3.5 24.655
KH2PO4 1 7.348
(NH4)2SO4 0.5 3.784
MgCl2 x 6 H2O 0.1 0.492
Ca(NO3)2 x 4 H2O 0.05 0.212
NaOH 5.0e-4 0.013
EDTA 5.0e-4 0.002
FeSO4 x 7 H2O 2.0e-4 7.2e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
alpha-lipoic acid 5.0e-5 2.4e-4
Pantothenic acid 5.0e-5 2.3e-4
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Solutions:
  • 1 ml Trace element solution SL-4
  • 1 ml Selenite/tungstate stock solution
  • 1 ml Vitamin solution
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