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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Porcine serum

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1078 FRIIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
PPLO broth 5.404 -
Bacto brain heart infusion 5.093 -
NaCl 2.484 42.513
Yeast extract 0.83 -
KCl 0.124 1.666
CaCl2 0.057 0.518
Na2HPO4 x 2 H2O 0.047 0.328
MgCl2 x 6 H2O 0.031 0.153
MgSO4 x 7 H2O 0.031 0.126
KH2PO4 0.019 0.137
Phenol red 0.001 0.004
Distilled water - -
Water - -
Porcine serum - -
Solutions:
  • 50 ml Hank's BSS
  • 25 ml Solution 1
  • 25 ml Solution 2