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Current criteria: Potassium phosphate buffer

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156 ANAEROTIGNUM MEDIUM

Gas phase:
  • 100 % N2

461b MINERAL MEDIUM WITH PYRROLIC ACID

Compound Conc. [g/L] Conc. [mM]
Pyrrole-2-carboxylic acid 11 99.01
Na2HPO4 x 2 H2O 1.427 10.053
MgSO4 x 7 H2O 0.492 1.997
NH4Cl 0.295 5.52
KH2PO4 0.246 1.808
NaCl 0.049 0.842
CaCl2 0.01 0.089
MnSO4 0.01 0.065
HCl 0.002 0.067
FeCl2 x 4 H2O 0.001 0.007
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
Thiamine-HCl x 2 H2O 2.5e-4 6.6e-4
Riboflavin 2.5e-4 6.5e-4
Vitamin B12 2.5e-4 1.8e-4
CoCl2 x 6 H2O 1.9e-4 0.001
Nicotine amide 1.2e-4 0.001
Nicotinic acid 1.2e-4 1.0e-3
MnCl2 x 4 H2O 9.8e-5 5.0e-4
Biotin 9.8e-5 4.0e-4
Folic acid 9.8e-5 2.2e-4
ZnCl2 6.9e-5 5.1e-4
Pyridoxamine hydrochloride 4.9e-5 2.4e-4
Na2MoO4 x 2 H2O 3.5e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 5.9e-6 9.5e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Potassium phosphate buffer - 0.495
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Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10
  • 10 ml Pyrrole-2-carboxylic acid solution

746 RHODOVULUM STRICTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 8.2 140.315
Na-DL-malate 3.6 20.219
(NH4)2SO4 1 7.568
Yeast extract 1 -
Na2S2O3 x 5 H2O 0.5 2.015
MgCl2 x 6 H2O 0.2 0.984
CaCl2 x 2 H2O 0.05 0.34
Na2-EDTA 0.005 0.015
FeCl2 x 4 H2O 0.002 0.008
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
H3BO3 6.2e-5 0.001
Na2MoSO4 x 2 H2O 3.6e-5 -
NiCl2 x 6 H2O 2.4e-5 1.9e-4
CuCl2 x 2 H2O 1.7e-5 1.0e-4
Potassium phosphate buffer - 5
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Solutions:
  • 500 ml Solution 1
  • 500 ml Solution 2
  • 1 ml Trace element solution SL-8

828 NEVSKIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Potassium phosphate buffer 64.35 -
Agar 15 44.599
Na-lactate 0.56 4.997
MgSO4 x 7 H2O 0.05 0.203
CaCl2 x 2 H2O 0.014 0.095
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Solutions:
  • 0.5 ml SL-9
  • 0.5 ml Seven vitamins solution

833 DEHALOSPIRILLUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 6.381 39.871
Na-pyruvate 4.487 40.771
Yeast extract 1.994 -
Na2CO3 1.745 16.462
Na2SO4 0.698 4.913
KCl 0.499 6.687
MgCl2 x 6 H2O 0.399 1.962
NH4Cl 0.249 4.66
NaCl 0.249 4.265
KH2PO4 0.199 1.465
CaCl2 x 2 H2O 0.15 1.017
L-Cysteine HCl x H2O 0.05 0.284
FeSO4 x 7 H2O 0.013 0.046
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
D-(+)-biotin 2.0e-5 8.2e-5
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Potassium phosphate buffer - 0.01
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Solutions:
  • 892 ml Solution A
  • 10 ml Solution B
  • 2 ml Solution C
  • 35 ml Solution D
  • 20 ml Solution E
  • 40 ml Solution F
  • 3 ml Solution G
  • 1 ml Solution H
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
  • 1 ml Seven vitamins solution
Gas phase:
  • 100 % N2

J915 SEMI-SOLID AUTOTROPHIC GROWTH MEDIUM FOR MAGNETOCOCCUS MARINUS

Compound Conc. [g/L] Conc. [mM]
NaCl 16.094 275.397
MgCl2 x 6 H2O 3.425 16.846
Na2SO4 2.689 18.931
Agarose 1.963 3.113
KCl 0.461 6.187
CaCl2 x 2 H2O 0.383 2.603
NH4Cl 0.294 5.504
Thiamine HCl 4.4e-4 -
Resazurin 3.9e-4 0.002
Myo-inositol 2.0e-4 0.001
p-Aminobenzoic acid 2.5e-5 1.8e-4
Vitamin B12 2.5e-5 1.8e-5
Nicotinic acid 2.0e-5 1.6e-4
Calcium pantothenate 2.0e-5 4.1e-5
Pyridoxine hydrochloride 2.0e-5 -
Folic acid 9.8e-6 2.2e-5
Biotin 4.9e-7 2.0e-6
L-Cysteine HCl x H2O - -
NaHCO3 - -
Distilled water - -
Na2S2O3 x 5 H2O - -
Potassium phosphate buffer - -
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Solutions:
  • 5 ml Modified Wolfe's mineral solution
  • 0.5 ml Vitamin solution

J918 DESULFAMPLUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 19.455 332.91
MgCl2 x 6 H2O 5.837 28.709
Na2SO4 3.152 22.189
HEPES 2.335 9.797
CaCl2 x 2 H2O 0.973 6.617
Sodium fumarate 0.973 6.078
KCl 0.535 7.177
NH4Cl 0.292 5.456
Yeast extract 0.195 -
Thiamine HCl 4.4e-4 -
Resazurin 3.9e-4 0.002
Myo-inositol 1.9e-4 0.001
p-Aminobenzoic acid 2.4e-5 1.8e-4
Vitamin B12 2.4e-5 1.8e-5
Nicotinic acid 1.9e-5 1.6e-4
Calcium pantothenate 1.9e-5 4.1e-5
Pyridoxine hydrochloride 1.9e-5 -
Folic acid 9.7e-6 2.2e-5
Biotin 4.9e-7 2.0e-6
NaHCO3 - -
L-Cysteine HCl x H2O - -
Distilled water - -
Potassium phosphate buffer - -
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Solutions:
  • 5 ml Modified Wolfe's mineral solution
  • 0.5 ml Vitamin solution

J1205 DESULFOVIBRIO FSS-1 MEDIUM

Solutions:
  • 5 ml Modified Wolfe's mineral solution
  • 5 ml Vitamin solution