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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Pyridoxine

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857 BACTO MIDDLEBROOK 7H9 BROTH

Compound Conc. [g/L] Conc. [mM]
Bovine albumin 5 -
Disodium Phosphate 2.5 17.611
Dextrose 2 10.092
Monopotassium Phosphate 1 7.348
Ammonium Sulfate 0.5 3.784
L-Glutamic acid 0.5 3.398
Sodium citrate 0.1 0.387
Magnesium sulfate 0.05 0.415
Ferric ammonium citrate 0.04 -
Copper Sulfate 1.0e-3 0.006
Zinc Sulfate 1.0e-3 0.006
Pyridoxine 1.0e-3 0.006
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Solutions:
  • 100 ml ADC Enrichment

888 AQUASPIRILLUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Casamino acids 1.5 -
(NH4)2SO4 1 7.568
Sodium succinate 1 6.171
MgSO4 x 7 H2O 1 4.057
Agar 0.5 1.487
Na2S2O3 x 5 H2O 0.1 0.403
CaCl2 x 6 H2O 0.03 0.137
Na2HPO4 0.01 0.07
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.008
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
H3BO3 3.0e-5 4.9e-4
NiCl2 x 6 H2O 3.0e-5 2.3e-4
CuCl2 x 2 H2O 3.0e-5 1.8e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
Pyridoxine 2.5e-5 1.5e-4
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Solutions:
  • 1 ml Trace elements solution (Pfennig & Lippert,1966)
  • 1 ml Vitamine mixture
  • 100 ml Acidic thiamine / Ca pantothenate solution
  • 100 ml Basic vitamin solution
  • 100 ml Neutral folic acid / riboflavin solution
  • 100 ml Neutral B12 solution 0.005 g/L

911 MIDDLEBROCK 7H11 AGAR

Compound Conc. [g/L] Conc. [mM]
Bovine albumin 5 -
Dextrose 2 10.092
NaCl 0.85 14.545
Agar 0.35 1.041
Oleic acid 0.05 0.177
KH2PO4 0.035 0.257
Na2HPO4 0.035 0.247
Pancreatic digest of casein 0.023 -
(NH4)2SO4 0.012 0.088
L-Glutamic acid 0.012 0.079
Sodium citrate 0.009 0.036
Catalase 0.004 -
MgSO4 0.001 0.01
Ferric ammonium citrate 9.3e-4 -
Pyridoxine 2.3e-5 1.4e-4
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Solutions:
  • 21 ml Bacto Middelbrook 7H11 agar (Difco 283810)
  • 100 ml Bacto Middelbrook OADC enrichment (Difco 212240):

929 RHODOVULUM IODOSUM AND R. ROBIGINOSUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 26.4 451.745
MgSO4 x 7 H2O 6.8 27.589
MgCl2 x 6 H2O 5.7 28.037
NaHCO3 2.52 29.998
FeSO4 1.519 10
CaCl2 x 2 H2O 1.5 10.203
KCl 0.66 8.853
KH2PO4 0.4 2.939
Acetate 0.295 5
NH4Cl 0.25 4.674
Na2S2O3 0.214 0.5
KBr 0.09 0.756
Na2-EDTA 0.005 0.015
FeSO4 x 7 H2O 0.002 0.008
NaOH 2.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
Pyridoxin 1.5e-4 8.9e-4
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Solutions:
  • 1 ml Trace elements
  • 1 ml Selenite/tungsate solution
  • 1 ml Vitamin solution
  • 1 ml Vitamin B12 solution 0.05 g/L
  • 1 ml Vitamin B1 solution 0.1 g/L
Gas phase:
  • 80 % N2
  • 20 % CO2

1154 GLUCOSE SULFIDE MEDIUM (Richard et al., 1985)

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
(NH4)2SO4 0.5 3.784
Na2S x 9 H2O 0.187 0.779
Glucose 0.15 0.833
CaCO3 0.1 0.999
Ca(NO3)2 0.1 0.609
KCl 0.05 0.671
K2HPO4 0.05 0.287
MgSO4 x 7 H2O 0.05 0.203
Biotin 6.0e-9 2.5e-8
Folic acid 6.0e-9 1.4e-8
Cyanocobalamin 6.0e-9 4.4e-9
Niacin 1.0e-10 8.1e-10
p-Aminobenzoic acid 1.0e-10 7.3e-10
Pyridoxine 1.0e-10 5.9e-10
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Solutions:
  • 1 ml Vitamin solution

1398 1/10 PYGV MEDIUM modified

Compound Conc. [g/L] Conc. [mM]
Glucose 1 5.551
Trypticase 1 -
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
KH2PO4 0.45 3.307
K2HPO4 0.45 2.584
CaCl2 x 2 H2O 0.09 0.612
MgSO4 x 7 H2O 0.09 0.365
Pyridoxine 0.015 0.089
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Solutions:
  • 75 ml S I
  • 75 ml S II
  • 10 ml 100x Vitamin solution

1464 CHITINIVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 95 896.328
NaHCO3 15 178.557
NaCl 6 102.669
Chitin 1.5 -
K2HPO4 1 5.741
NH4Cl 0.214 4
MgSO4 0.12 1
Na2S 0.079 1
Yeast extract 0.02 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
Pyridoxine 3.3e-5 2.0e-4
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Solutions:
  • 1 ml Vitamine mixture (Pfennig & Lippert, 1965)
  • 1 ml Acidic trace metals (Pfennig & Lippert, 1965)
  • 50 ml Acidic solution (HCl, pH 3; 100 ml)
  • 50 ml Basic solution (100 ml 0.1 N NaOH)
  • 50 ml Neutral solution (100 ml Aqua dem.)

1465 CHITINISPIRILLUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 22 207.571
NaHCO3 8 95.23
NaCl 6 102.669
Chitin 1.5 -
K2HPO4 1 5.741
NH4Cl 0.214 4
MgSO4 0.12 1
Na2S 0.079 1
Yeast extract 0.02 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
Pyridoxine 3.3e-5 2.0e-4
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Solutions:
  • 1 ml Acidic trace metals (Pfennig & Lippert, 1965)
  • 1 ml Vitamine mixture (Pfennig & Lippert, 1965)
  • 50 ml Acidic solution (HCl, pH 3; 100 ml)
  • 50 ml Basic solution (100 ml 0.1 N NaOH)
  • 50 ml Neutral solution (100 ml Aqua dem.)

1478 NATRANAEROARCHAEUM MEDIUM AARC1

Compound Conc. [g/L] Conc. [mM]
Na2CO3 142.5 1344.49
NaCl 72 1232.03
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
MgCl2 x 6 H2O 0.244 1.2
Sodium acetate 0.164 2
NH4Cl 0.16 3
(NH4)2SO4 0.125 0.946
NaHCO3 0.105 1.25
Yeast extract 0.02 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 1.0e-4 6.2e-4
MnCl2 3.0e-5 2.4e-4
Pyridoxine 2.5e-5 1.5e-4
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamine mixture
  • 100 ml Acidic thiamine / Ca pantothenate solution
  • 100 ml Basic vitamin solution
  • 100 ml Neutral folic acid / riboflavin solution
  • 100 ml Neutral B12 solution 0.005 g/L

1479 HALODESULFURIARCHAEUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 240 4106.78
HEPES 10 41.964
Sodium formate 3.4 50
K2HPO4 2.5 14.353
Sulfur powder 2 7.797
MgCl2 x 6 H2O 0.61 3
(NH4)2SO4 0.5 3.784
NaHCO3 0.42 5
Yeast extract 0.2 -
EDTA 0.005 0.017
FeSO4 x 7 H2O 0.002 0.007
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 1.0e-4 6.2e-4
MnCl2 3.0e-5 2.4e-4
Pyridoxine 2.5e-5 1.5e-4
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Solutions:
  • 1 ml Trace element solution
  • 1 ml Vitamin mix (pfennig & Lippert,1966)
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