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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Quinic acid

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380 MAGNETOSPIRILLUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		1.3 3.865
KH2PO4 0.68 4.997
Succinic acid 0.37 3.133
L(+)-Tartaric acid 0.37 2.465
NaNO3 0.12 1.412
Yeast extract 0.1 -
Na-acetate 0.05 0.61
Na-thioglycolate 0.05 0.438
MgSO4 x 7 H2O 0.015 0.061
FeCl3 x 6 H2O 0.009 0.033
Nitrilotriacetic acid 0.008 0.039
NaCl 0.005 0.086
Quinic acid 0.004 0.02
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Solutions:
  • 5 ml Modified Wolin's mineral solution
  • 2 ml Fe(III) quinate solution 0.01 M
  • 1 ml Seven vitamins solution
Gas phase:
  • 99 % N2
  • 1 % O2

380a TELMATOSPIRILLUM (PEAT) MEDIUM

Compound Conc. [g/L] Conc. [mM]
D-Xylose 0.992 6.608
KH2PO4 0.675 4.957
Succinic acid 0.367 3.108
L(+)-Tartaric acid 0.367 2.446
NaNO3 0.119 1.401
Na-acetate 0.05 0.605
Na-thioglycolate 0.05 0.435
MgSO4 x 7 H2O 0.015 0.06
FeCl3 x 6 H2O 0.009 0.033
Nitrilotriacetic acid 0.007 0.039
NaCl 0.005 0.085
Quinic acid 0.004 0.02
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Solutions:
  • 5 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
  • 2 ml Fe(III) quinate solution 0.01 M
Gas phase:
  • 99 % N2
  • 1 % O2

896 SOLIDESULFOVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2-fumarate 0.576 3.599
Na-pyruvate 0.437 3.971
KH2PO4 0.199 1.459
NH4Cl 0.06 1.114
L-Cysteine HCl x H2O 0.05 0.283
MgSO4 x 7 H2O 0.012 0.048
FeCl3 x 6 H2O 0.009 0.033
Nitrilotriacetic acid 0.006 0.031
NaCl 0.004 0.068
Quinic acid 0.004 0.02
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Solutions:
  • 2 ml Fe(III) quinate solution 0.01 M
  • 4 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2

J399 DESULFOVIBRIO MAGNETICUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sodium fumarate 0.572 3.574
Sodium pyruvate 0.434 3.943
K2HPO4 0.197 1.132
NH4Cl 0.059 1.106
L-Cysteine HCl x H2O 0.049 -
MgSO4 x 7 H2O 0.012 0.048
FeCl3 x 6 H2O 0.009 0.033
Nitrilotriacetic acid 0.006 0.031
NaCl 0.004 0.068
Quinic acid 0.004 0.02
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Solutions:
  • 2 ml Fe(III) quinate solution
  • 4 ml Trace minerals
  • 8 ml Trace vitamins

J401 PROPIONIVIBRIO MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 192.308 2289.19
NaCl 77 1317.59
2,3-butanediol 69.231 768.206
KCl 38.462 515.916
MgCl2 x 6 H2O 30.769 151.347
Na2S x 9 H2O 27.692 115.297
NH4Cl 19.231 359.521
KH2PO4 15.385 113.051
CaCl2 x 2 H2O 11.546 78.537
MgSO4 x 7 H2O 0.231 0.936
Nitrilotriacetic acid 0.115 0.604
Resazurin 0.077 0.336
MnSO4 x n H2O 0.038 -
CoSO4 x 7 H2O 0.008 0.05
FeSO4 x 7 H2O 0.008 0.028
ZnSO4 x 7 H2O 0.008 0.027
Pyridoxine hydrochloride 0.008 -
Nicotinic acid 0.004 0.031
p-Aminobenzoic acid 0.004 0.028
Lipoic acid 0.004 0.019
Riboflavin 0.004 0.01
Calcium pantothenate 0.004 0.008
Thiamine HCl 0.004 -
Biotin 0.002 0.006
Folic acid 0.002 0.003
H3BO3 7.7e-4 0.012
Na2MoO4 x 2 H2O 7.7e-4 0.003
CuSO4 x 5 H2O 7.7e-4 0.003
AlK(SO4)2 7.7e-4 0.003
Vitamin B12 7.7e-5 5.7e-5
Quinic acid - -
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Solutions:
  • 1 ml Trace minerals
  • 10 ml Trace vitamins

J669 MAGNETOSPIRILLUM MEDIUM (B)

Compound Conc. [g/L] Conc. [mM]
Sodium succinate 0.365 2.254
Sodium tartrate 0.365 1.882
KH2PO4 0.276 2.031
NaNO3 0.118 1.394
Sodium acetate 0.049 0.602
Sodium thioglycolate 0.049 0.433
MgSO4 x 7 H2O 0.029 0.119
Nitrilotriacetic acid 0.01 0.052
FeCl3 x 6 H2O 0.009 0.033
Quinic acid 0.004 0.02
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Solutions:
  • 50 ml Metals ''44''
  • 10 ml Trace vitamins
  • 2 ml Ferric quinate solution
  • 1 ml Mineral salt solution