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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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461a MINERAL MEDIUM WITH QUINOLINE

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 1.31 9.23
MgSO4 x 7 H2O 0.452 1.833
Quinoline 0.273 2.112
NH4Cl 0.271 5.068
KH2PO4 0.226 1.66
NaCl 0.045 0.773
CaCl2 0.009 0.081
MnSO4 0.009 0.06
HCl 0.002 0.062
FeCl2 x 4 H2O 0.001 0.007
p-Aminobenzoic acid 2.3e-4 0.002
alpha-lipoic acid 2.3e-4 0.001
Pantothenic acid 2.3e-4 0.001
Thiamine-HCl x 2 H2O 2.3e-4 6.1e-4
Riboflavin 2.3e-4 6.0e-4
Vitamin B12 2.3e-4 1.7e-4
CoCl2 x 6 H2O 1.7e-4 0.001
Nicotine amide 1.1e-4 9.2e-4
Nicotinic acid 1.1e-4 9.2e-4
MnCl2 x 4 H2O 9.0e-5 4.6e-4
Biotin 9.0e-5 3.7e-4
Folic acid 9.0e-5 2.0e-4
ZnCl2 6.3e-5 4.6e-4
Pyridoxamine hydrochloride 4.5e-5 2.2e-4
Na2MoO4 x 2 H2O 3.3e-5 1.3e-4
NiCl2 x 6 H2O 2.2e-5 1.7e-4
H3BO3 5.4e-6 8.8e-5
CuCl2 x 2 H2O 1.8e-6 1.1e-5
Phosphate buffer - 4.545
Distilled water - -
Solutions:
  • 5 ml Vitamin solution
  • 1 ml Trace element solution SL-10
  • 100 ml Quinoline solution