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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Sludge

Your search received 7 results.

119 METHANOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Isobutyric acid - -
DL-2-Methylbutyric acid - -
Valeric acid - -
Isovaleric acid - -
Distilled water - -
H2SO4 - -
Sludge - -
Solutions:
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
H2SO4 - -
DL-2-Methylbutyric acid - -
Isovaleric acid - -
Valeric acid - -
Sludge - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

119c GUDONGELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 2 29.409
Na-acetate 1 12.19
NaHCO3 0.5 5.952
KH2PO4 0.5 3.674
L-Cysteine HCl x H2O 0.5 2.847
Na2S x 9 H2O 0.5 2.082
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
HCl 0.003 0.069
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.002 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Valeric acid - -
DL-2-Methylbutyric acid - -
Isobutyric acid - -
Sludge - -
Show more
Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 100 % N2

140 RUMINICLOSTRIDIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
Na2CO3 2.5 23.588
Yeast extract 1.2 -
NaCl 1 17.112
(NH4)2SO4 0.5 3.784
KH2PO4 0.5 3.674
K2HPO4 0.5 2.871
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
CaCl2 x 2 H2O 0.1 0.68
MgSO4 x 7 H2O 0.1 0.406
Sodium resazurin 5.0e-4 -
Sludge - -
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Solutions:
  • 300 ml Sludge fluid
  • 300 ml Clarified rumen fluid
Gas phase:
  • 100 % CO2

320 CLOSTRIDIUM CELLULOVORANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Cellobiose 5 14.607
NH4Cl 1 18.695
Na2CO3 1 9.435
K2HPO4 x 3 H2O 1 5.741
KCl 0.5 6.707
MgSO4 x 7 H2O 0.5 2.029
Trypticase peptone 0.5 -
L-Cysteine HCl x H2O 0.15 0.854
Na2S x 9 H2O 0.15 0.625
Yeast extract 0.08 -
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
Sodium resazurin 5.0e-4 -
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
Sludge - -
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Solutions:
  • 20 ml Sludge fluid
  • 1 ml Trace element solution SL-10
  • 20 ml Clarified rumen fluid
Gas phase:
  • 80 % N2
  • 20 % CO2

321 METHANOCORPUSCULUM AGGREGANS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 5 73.522
Na2CO3 1.5 14.153
NH4Cl 1 18.695
Na-acetate 1 12.19
Trypticase peptone 1 -
K2HPO4 x 3 H2O 0.4 2.297
MgCl2 x 6 H2O 0.4 1.968
(NH4)2SO4 0.3 2.27
KH2PO4 0.3 2.205
L-Cysteine HCl x H2O 0.2 1.139
Na2S x 9 H2O 0.2 0.833
MgSO4 x 7 H2O 0.03 0.122
Yeast extract 0.02 -
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Distilled water - -
Sludge - -
Solutions:
  • 50 ml Minerals solution
  • 10 ml Modified Wolin's mineral solution
  • 5 ml Clarified rumen fluid
  • 5 ml Sludge fluid
Gas phase:
  • 80 % H2
  • 20 % CO2

1084 METHERMICOCCUS (METHANOGEN) MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 23.506 402.231
Methanol 12.411 387.347
MgCl2 x 6 H2O 9.99 49.14
2-Mercaptoethanesulfonic acid 2.449 17.219
Na2CO3 0.979 9.241
KCl 0.333 4.467
L-Cysteine HCl x H2O 0.294 1.673
Na2S x 9 H2O 0.294 1.223
NH4Cl 0.245 4.578
K2HPO4 0.196 1.125
Yeast extract 0.02 -
Sodium resazurin 4.9e-4 0.002
Sludge - -
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Solutions:
  • 5 ml Sludge fluid
Gas phase:
  • 80 % N2
  • 20 % CO2