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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Sodium bicarbonate
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1486 GBS salts modified for Thermocrinis jamiesonii
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| NaCl | 3 | 51.335 |
| NaH2PO4 | 1.38 | 11.502 |
| Sodium thiosulfate pentahydrate | 0.496 | 2 |
| Na2SO4 | 0.3 | 2.112 |
| Sodium acetate trihydrate | 0.272 | 2 |
| KCl | 0.15 | 2.012 |
| MgSO4 x 7 H2O | 0.123 | 0.499 |
| NH4Cl | 0.107 | 2 |
| Na2-EDTA | 0.019 | 0.055 |
| CaCl2 x 2 H2O | 0.015 | 0.102 |
| FeSO4 x 7 H2O | 0.006 | 0.02 |
| MnCl2 x 4 H2O | 4.9e-4 | 0.002 |
| H3BO3 | 1.5e-4 | 0.003 |
| ZnSO4 x 7 H2O | 1.4e-4 | 5.0e-4 |
| Na2MoO4 x 2 H2O | 1.2e-4 | 5.0e-4 |
| CoCl2 x 6 H2O | 1.2e-4 | 9.2e-4 |
| CuCl2 x 2 H2O | 8.6e-5 | 5.0e-4 |
| Sodium bicarbonate | - | 1 |
| Distilled water | - | - |
Solutions:
- 5 ml Mineral solution
1829 BSKII medium (Borrelia)
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| MEM alpha Modification | 8.9 | - |
| Bovine serum albumin | 8.5 | - |
| HEPES | 5.1 | 21.402 |
| Glucose | 4.2 | 23.313 |
| Bacto peptone | 4.2 | - |
| Sodium bicarbonate | 1.8 | - |
| Yeast extract | 1.7 | - |
| Pyruvate | 0.6 | 6.893 |
| Trisodium citrate x 2 H2O | 0.5 | 1.7 |
| N-Acetylglucosamine | 0.3 | 1.356 |
| Rabbit serum | - | - |
| Deionized water | - | - |