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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.
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Current criteria: Succinate
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576 SUCCINATE MINERAL MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| K2HPO4 x 3 H2O | 1.63 | 9.358 |
| Succinate | 1.161 | 10 |
| NH4Cl | 1.02 | 19.069 |
| KH2PO4 | 0.39 | 2.866 |
| MgSO4 x 7 H2O | 0.062 | 0.252 |
| Trace element solution | - | - |
| Distilled water | - | - |
Solutions:
- 1 ml Trace element solution
- 1000 ml Solution A
- 1000 ml Solution B
1401 MSYS MEDIUM
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Succinate | 1 | 8.615 |
| (NH4)2SO4 | 1 | 7.568 |
| KH2PO4 | 1 | 7.348 |
| Malate | 1 | - |
| Yeast extract | 0.5 | - |
| NaCl | 0.2 | 3.422 |
| MgCl2 x 6 H2O | 0.2 | 0.984 |
| CaCl2 x 2 H2O | 0.045 | 0.306 |
| Na2-EDTA | 0.005 | 0.015 |
| FeCl2 x 4 H2O | 1.0e-3 | 0.005 |
| H3BO3 | 3.0e-4 | 0.005 |
| CoCl2 x 6 H2O | 1.1e-4 | 8.5e-4 |
| ZnCl2 | 5.0e-5 | 3.7e-4 |
| NiSO4 x 6 H2O | 3.0e-5 | 1.9e-4 |
| Na2MoO4 x 2 H2O | 3.0e-5 | 1.2e-4 |
| MnCl2 x 2 H2O | 3.0e-5 | - |
| CuCl2 x 2 H2O | 1.0e-5 | 5.9e-5 |
| Distilled water | - | - |
Solutions:
- 1 ml Trace elements solution
Gas phase:
- 100 % N2