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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.



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Current criteria: Sulphur

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806 THERMOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulphur 5 -
MgCl2 x 2 H2O 2.7 20.573
NaHCO3 1 11.904
Trypticase 1 -
Yeast extract 1 -
L-Cysteine HCl x H2O 0.5 2.847
Na2S x 9 H2O 0.5 2.082
KCl 0.33 4.427
NH4Cl 0.25 4.674
K2HPO4 0.14 0.804
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
Resazurin 1.0e-3 0.004
FeSO4 x 7 H2O 1.0e-3 0.004
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Na2SeO3 1.0e-6 5.8e-6
Distilled water - -
Solutions:
  • 10 ml Modified Wolin's mineral solution II
  • 10 ml Wolin's vitamin solution

934 THERMOCOCCUS WAIOTAPUENSE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sulphur 10 -
MgCl2 5.8 60.918
Tryptone 5 70.343
Na2SO4 4 28.161
NaCl 1.5 25.667
CaCl2 1.5 13.516
Yeast extract 1 -
Na2S x 9 H2O 0.75 3.123
KCl 0.7 9.39
NaHCO3 0.2 2.381
KBr 0.1 0.84
H3BO3 0.03 0.485
SrCl2 0.025 0.158
Resazurin 0.002 0.009
Distilled water - -
Gas phase:
  • 100 % N2