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Current criteria: Thiamine hydrochloride

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78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

113a SULFURIMONAS HONGKONGENSIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 24.9 426.085
Na2S2O3 x 5 H2O 4.98 20.066
KNO3 1.992 19.703
KH2PO4 1.992 14.638
Na2CO3 0.996 9.397
NH4Cl 0.996 18.621
MgSO4 x 7 H2O 0.797 3.233
FeSO4 x 7 H2O 0.002 0.007
Na2-EDTA 1.0e-3 0.003
H3BO3 6.0e-4 0.01
CoCl2 x 6 H2O 4.0e-4 0.003
ZnSO4 x 7 H2O 2.0e-4 6.9e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
MnCl2 x 4 H2O 6.0e-5 3.0e-4
Na2MoO4 x 2 H2O 6.0e-5 2.5e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
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Solutions:
  • 942 ml Solution A
  • 40 ml Solution B
  • 20 ml Solution C
  • 1 ml Solution D
  • 1 ml Solution E
  • 2 ml Trace element solution SL-4
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2

120 METHANOSARCINA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaCl 2.197 37.599
NaHCO3 1.953 23.25
Casitone 1.953 -
Yeast extract 1.953 -
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
K2HPO4 0.342 1.962
L-Cysteine HCl x H2O 0.293 1.668
Na2S x 9 H2O 0.293 1.22
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
Sodium resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.8e-5 4.9e-4
Pyridoxine hydrochloride 9.8e-5 4.7e-4
ZnCl2 6.8e-5 5.0e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.0e-4
Thiamine HCl 4.9e-5 1.4e-4
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 100 % N2
  • 20 % CO2

120a METHANOSARCINA BARKERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NaHCO3 0.83 9.881
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
K2HPO4 0.342 1.962
L-Cysteine HCl x H2O 0.293 1.668
Na2S x 9 H2O 0.293 1.22
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
Sodium resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.8e-5 4.9e-4
Pyridoxine hydrochloride 9.8e-5 4.7e-4
ZnCl2 6.8e-5 5.0e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.0e-4
Thiamine HCl 4.9e-5 1.4e-4
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

120b METHANOMICROCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 15.469 482.765
Na-acetate 2.441 29.761
NaHCO3 2.441 29.062
NaCl 2.197 37.599
Yeast extract 1.953 -
Casitone 1.953 -
NH4Cl 0.488 9.128
MgSO4 x 7 H2O 0.488 1.981
L-Cysteine HCl x H2O 0.352 2.002
Na2S x 9 H2O 0.352 1.464
K2HPO4 0.342 1.962
CaCl2 x 2 H2O 0.244 1.661
KH2PO4 0.225 1.651
2-Mercaptoethanesulfonate 0.137 0.961
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
Sodium resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.8e-5 4.9e-4
Pyridoxine hydrochloride 9.8e-5 4.7e-4
ZnCl2 6.8e-5 5.0e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.0e-4
Thiamine HCl 4.9e-5 1.4e-4
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

120c METHANOSARCINA MAZEI (GOE1) MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sucrose 17.032 49.757
Na-acetate 9.96 121.415
NaCl 2.241 38.348
NaHCO3 1.992 23.713
Casitone 1.992 -
Yeast extract 1.992 -
NH4Cl 0.498 9.31
MgSO4 x 7 H2O 0.498 2.021
K2HPO4 0.349 2.001
L-Cysteine HCl x H2O 0.299 1.701
Na2S x 9 H2O 0.299 1.244
CaCl2 x 2 H2O 0.249 1.694
KH2PO4 0.229 1.683
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
ZnCl2 7.0e-5 5.1e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

120d METHANOSARCINA MARINE MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 22.461 384.342
Methanol 15.469 482.765
MgCl2 x 6 H2O 9.766 48.035
Na2CO3 0.977 9.214
NH4Cl 0.488 9.128
K2HPO4 0.342 1.962
L-Cysteine HCl x H2O 0.293 1.668
Na2S x 9 H2O 0.293 1.22
KH2PO4 0.225 1.651
CaCl2 x 2 H2O 0.146 0.996
HCl 0.002 0.067
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.007
Sodium resazurin 4.9e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.8e-5 4.9e-4
Pyridoxine hydrochloride 9.8e-5 4.7e-4
ZnCl2 6.8e-5 5.0e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.0e-4
Thiamine HCl 4.9e-5 1.4e-4
Show more
Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % N2
  • 20 % CO2

124 DESULFOFARCIMEN MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.487 53.407
Na2SO4 2.832 19.935
Na-acetate 1.396 17.015
Na-butyrate 1.396 12.679
NaCl 1.167 19.961
Yeast extract 0.997 -
MgCl2 x 6 H2O 0.399 1.962
Na2S x 9 H2O 0.399 1.66
KCl 0.299 4.012
NH4Cl 0.269 5.033
KH2PO4 0.199 1.465
CaCl2 x 2 H2O 0.15 1.017
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
ZnCl2 7.0e-5 5.1e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Show more
Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135 ACETOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 9.794 116.589
D-Fructose 9.794 54.365
Yeast extract 1.959 -
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Sodium resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
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Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2

135a ACETOBACTERIUM MEDIUM (MARINE)

Compound Conc. [g/L] Conc. [mM]
NaHCO3 4.897 58.295
Yeast extract 1.959 -
Ethylene glycol 1.224 19.725
NH4Cl 0.979 18.311
L-Cysteine HCl x H2O 0.49 2.788
Na2S x 9 H2O 0.49 2.039
K2HPO4 0.441 2.53
KH2PO4 0.323 2.375
MgSO4 x 7 H2O 0.059 0.238
Nitrilotriacetic acid 0.029 0.154
NaCl 0.02 0.335
MnSO4 x H2O 0.01 0.058
CoSO4 x 7 H2O 0.004 0.023
ZnSO4 x 7 H2O 0.004 0.012
CaCl2 x 2 H2O 0.002 0.013
FeSO4 x 7 H2O 0.002 0.007
NiCl2 x 6 H2O 5.9e-4 0.005
Resazurin 4.9e-4 0.002
AlK(SO4)2 x 12 H2O 3.9e-4 8.3e-4
H3BO3 2.0e-4 0.003
Na2MoO4 x 2 H2O 2.0e-4 8.1e-4
CuSO4 x 5 H2O 2.0e-4 7.8e-4
Pyridoxine hydrochloride 9.8e-5 4.8e-4
Nicotinic acid 4.9e-5 4.0e-4
p-Aminobenzoic acid 4.9e-5 3.6e-4
(DL)-alpha-Lipoic acid 4.9e-5 2.4e-4
Calcium D-(+)-pantothenate 4.9e-5 2.1e-4
Thiamine HCl 4.9e-5 1.5e-4
Show more
Solutions:
  • 20 ml Modified Wolin's mineral solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2
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