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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Thymidine

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394 CHI 1776 MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 23.365 328.707
Yeast extract 7.009 -
Glucose 5 27.753
MgCl2 0.476 5
Diaminopimelic acid 0.1 0.526
Thymidine 0.04 0.165
Tris-HCl - 20
Distilled water - -

1494 . OMIZ-PAT Medium (modified)

Compound Conc. [g/L] Conc. [mM]
(NH4)6Mo7O24 x 4 H2O 12.4 -
Yeast extract 3 -
Fe SO4 x 7 H2O 2.78 10
D-Arabinose 2 13.322
L-Rhamnose 2 12.183
L-Fucose 2 12.183
D-Mannose 2 11.102
D-Glucose 2 11.101
D-Trehalose 2 5.843
D-Sucrose 2 5.843
NH4Cl 1.6 29.912
Glutathione 1.537 5
Ethanol 1 21.706
NaHCO3 1 11.904
Ascorbic acid 1 5.678
KCl 0.968 12.985
D-Fructose 0.8 4.441
D-Mannitol 0.8 4.392
D-glucuronic acid 0.8 4.121
D-galacturonic acid 0.8 4.121
D-Maltose 0.8 2.337
L-Glutamine 0.68 4.653
L-Histidine 0.62 3.996
NaVO3 0.61 5.003
D 0.56 4.866
Pyruvic acid 0.55 6.246
L-Serine 0.525 4.996
Fumaric acid 0.5 4.308
HCl 0.365 10
L-Cysteine HCl 0.35 2.221
Formic acid 0.3 6.518
L-Glutamic acid 0.3 2.039
ZnSO4 x 7 H2O 0.287 0.998
MgSO4 x 7 H2O 0.25 1.014
DL-carnitine 0.2 1.241
Citric acid 0.2 1.041
N-Acetylglucosamine 0.2 0.904
L-Lysine HCl 0.182 -
L-Arginine 0.175 1.005
L-Ornithine HCl 0.17 -
L-Phenylalanine 0.165 0.999
CaCl2 x H2O 0.15 1.352
L-Asparagine 0.15 1.135
L-Methionine 0.15 1.005
NaH2PO4 x H2O 0.14 1.167
L-Isoleucine 0.131 0.999
L-Leucine 0.131 0.999
NiSO4 x 6 H2O 0.131 0.846
L-Threonine 0.12 1.007
L-Aspartic acid 0.118 0.887
SnCl2 x 2 H2O 0.118 0.523
L-Valine 0.117 0.999
L-Proline 0.115 0.999
L-Tryptophan 0.102 0.499
Nicotinic acid 0.1 0.812
Folinic acid 0.1 0.211
L-Tyrosine 0.09 0.497
NaOH 0.08 2
Glycine 0.075 0.999
Nicotinamide 0.05 0.409
Choline chloride 0.05 0.358
Myo-inositol 0.05 0.278
D-(+)-biotin 0.05 0.205
Folic acid 0.05 0.113
Vitamine B12 0.05 0.037
L-Alanine 0.045 0.505
Hemin 0.026 0.04
N-acetylmuramic acid 0.025 0.085
Na2SeO3 0.017 0.1
MnSO4 x H2O 0.017 0.1
2-Mercaptoethanesulfonic acid 0.01 0.07
Phenol red 0.01 0.028
Riboflavine 0.01 0.027
Pyridoxal hydrochloride 0.005 0.025
Pyridoxal phosphate 0.005 0.02
Thiamine HCl 0.005 0.015
Thiamin pyrophosphate 0.005 0.012
Calcium D-(+)-pantothenate 0.005 -
Putrescine 2 HCl 0.005 -
Thymidine 0.002 0.01
N-(2-acetamido)-2-aminoethanesulfonic acid 0.002 0.01
Hypoxanthine 0.001 0.01
Uracil 0.001 0.01
Coenzym A 1.0e-3 0.001
Flavin adenine dinucleotide 1.0e-3 0.001
ß-nicotinamide adenine dinucleotide 1.0e-3 -
CuSO4 7.9e-4 0.005
Cholesterol - -
Asialofetuin - -
DL-alpha-lipoic acid - -
2-Mercaptoethanol - -
Isovaleric acid - -
Valeric acid - -
2-Methylbutyric acid - -
Isobutyric acid - -
Distilled water - -
Rabbit serum - -
Solutions:
  • 200 ml Solution H
  • 8 ml Solution I
  • 2 ml Solution J
  • 40 ml Solution K
  • 2 ml Solution L
  • 200 ml Trace elements III
  • 10 ml Solution A
  • 10 ml Solution B
  • 10 ml Solution C
  • 10 ml Solution D
  • 10 ml Solution E
  • 10 ml Solution F
  • 10 ml Solution G
  • 2 ml Trace elements I
  • 2 ml Trace elements II
  • 200 ml Supplement 1
  • 2 ml Supplement 2
  • 2 ml Supplement 3
  • 2 ml Supplement 5
  • 200 ml Supplement 4