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Current criteria: Valeric acid

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78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
Riboflavin 5.9e-5 1.6e-4
Calcium D-(+)-pantothenate 5.9e-5 -
Biotin 2.3e-5 9.6e-5
Folic acid 2.3e-5 5.3e-5
Vitamin B12 1.2e-6 8.6e-7
NaOH - -
Vitamin K1 - -
Horse serum - -
Isobutyric acid - -
DL-2-Methylbutyric acid - -
Valeric acid - -
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

119 METHANOBACTERIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Isobutyric acid - -
DL-2-Methylbutyric acid - -
Valeric acid - -
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Solutions:
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
Gas phase:
  • 80 % H2
  • 20 % CO2

119a METHANOBREVIBACTER MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 3.988 47.473
Na-formate 1.994 29.321
Na-acetate 0.997 12.154
Yeast extract 0.997 -
KH2PO4 0.499 3.663
L-Cysteine HCl x H2O 0.499 2.838
Na2S x 9 H2O 0.499 2.076
NH4Cl 0.399 7.456
NaCl 0.399 6.824
MgSO4 x 7 H2O 0.399 1.618
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
H2SO4 - -
DL-2-Methylbutyric acid - -
Valeric acid - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 200 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 80 % H2
  • 20 % CO2

119b METHANOMASSILIICOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Methanol 7.888 246.191
Na-formate 1.992 29.292
Na-acetate 0.996 12.142
NaHCO3 0.996 11.856
KH2PO4 0.498 3.66
L-Cysteine HCl x H2O 0.498 2.835
Na2S x 9 H2O 0.498 2.073
NH4Cl 0.398 7.448
NaCl 0.398 6.817
MgSO4 x 7 H2O 0.398 1.616
Yeast extract 0.199 -
CaCl2 x 2 H2O 0.05 0.339
HCl 0.002 0.068
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.001 0.008
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.0e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
H3BO3 6.0e-6 9.7e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
H2SO4 - -
DL-2-Methylbutyric acid - -
Isovaleric acid - -
Valeric acid - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 66 % N2
  • 27 % H2
  • 7 % CO2

119c GUDONGELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na-formate 2 29.409
Na-acetate 1 12.19
NaHCO3 0.5 5.952
KH2PO4 0.5 3.674
L-Cysteine HCl x H2O 0.5 2.847
Na2S x 9 H2O 0.5 2.082
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
HCl 0.003 0.069
FeSO4 x 7 H2O 0.002 0.007
FeCl2 x 4 H2O 0.002 0.008
Sodium resazurin 5.0e-4 0.002
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
ZnCl2 7.0e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.9e-4
H3BO3 6.0e-6 9.7e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Valeric acid - -
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Solutions:
  • 2 ml FeSO4 x 7 H2O solution (0.1% w/v)
  • 1 ml Trace element solution SL-10
  • 50 ml Sludge fluid
  • 20 ml Fatty acid mixture
Gas phase:
  • 100 % N2

161 METHANOFOLLIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.998 23.784
Yeast extract 0.999 -
Trypticase 0.999 -
L-Cysteine HCl x H2O 0.5 2.844
Na2S x 9 H2O 0.5 2.08
(NH4)2SO4 0.3 2.268
KH2PO4 0.3 2.202
K2HPO4 0.3 1.721
MgSO4 x 7 H2O 0.03 0.122
Nitrilotriacetic acid 0.015 0.078
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.03
FeSO4 x 7 H2O 0.002 0.007
CoSO4 x 7 H2O 0.002 0.012
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
Sodium resazurin 5.0e-4 0.002
NiCl2 x 6 H2O 3.0e-4 0.002
AlK(SO4)2 x 12 H2O 2.0e-4 4.2e-4
H3BO3 1.0e-4 0.002
Pyridoxine hydrochloride 1.0e-4 4.9e-4
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Nicotinic acid 5.0e-5 4.1e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
Vitamin B12 1.0e-6 7.4e-7
Isovaleric acid - -
Valeric acid - -
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Solutions:
  • 300 ml Clarified rumen fluid
  • 10 ml Modified Wolin's mineral solution
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % H2
  • 20 % CO2

181 SELENOMONAS RUMINANTIUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Trypticase 5 -
Na-acetate 4 48.76
Na2CO3 4 37.74
Yeast extract 2 -
KH2PO4 1 7.348
Glucose 1 5.551
L-Cysteine HCl x H2O 0.5 2.847
Resazurin 1.0e-3 0.004
n-Valeric acid - -
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Gas phase:
  • 100 % CO2

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
Sucrose 1.688 4.93
Na2CO3 1.488 14.04
Trypticase peptone 0.992 -
Ethanol 0.942 20.457
D-Glucose 0.794 4.405
D-Xylose 0.744 4.956
Propionic acid 0.591 7.977
Na-pyruvate 0.496 4.508
KH2PO4 0.496 3.645
NH4Cl 0.397 7.419
NaCl 0.397 6.79
DL-Dithiothreitol 0.397 2.572
Starch 0.397 -
MgCl2 x 6 H2O 0.327 1.61
L-Cysteine HCl x H2O 0.298 1.695
iso-Butyric acid 0.094 1.069
n-Valeric acid 0.093 0.912
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

323 TREPONEMA SACCHAROPHILUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2CO3 2.5 23.588
D-Glucose 2 11.101
Trypticase peptone 2 -
Yeast extract 2 -
L-Cysteine HCl x H2O 1 5.694
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
KH2PO4 0.45 3.307
K2HPO4 0.45 2.584
MgSO4 x 7 H2O 0.19 0.771
CaCl2 x 2 H2O 0.12 0.816
Sodium resazurin 5.0e-4 -
n-Butyric acid - -
iso-Butyric acid - -
DL-2-Methylbutyric acid - -
n-Valeric acid - -
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Gas phase:
  • 100 % CO2

328 PYG MEDIUM WITH VOLATILE FATTY ACIDS

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Beef extract 5 -
Trypticase 5 -
(NH4)2SO4 0.5 3.784
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.019 0.078
CaCl2 x 2 H2O 0.012 0.082
Haemin 0.005 0.008
Resazurin 1.0e-3 0.004
Vitamin K1 1.0e-3 0.002
Distilled water - -
iso-Valeric acid - -
DL-2-Methylbutyric acid - -
n-Valeric acid - -
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Solutions:
  • 40 ml Mineral solution
  • 3.1 ml Fatty acid mixture
Gas phase:
  • 100 % N2
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