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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: Vitamin K1

Your search received 18 results.

78a CHOPPED MEAT MEDIUM FOR TREPONEMA SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 473.934 -
Casitone 28.436 -
Agar 14.218 42.274
K2HPO4 4.739 27.21
Yeast extract 4.739 -
L-Glutamine 0.664 4.54
L-Histidine 0.569 3.666
L-Serine 0.474 4.51
L-Cysteine HCl 0.474 3.007
Ethanol 0.18 3.909
Vitamin K3 0.047 -
Hemin 0.005 0.007
Resazurin 9.5e-4 0.004
p-Aminobenzoic acid 2.4e-4 0.002
alpha-lipoic acid 2.4e-4 0.001
Pantothenic acid 2.4e-4 0.001
Thiamine-HCl x 2 H2O 2.4e-4 6.3e-4
Riboflavin 2.4e-4 6.3e-4
Vitamin B12 2.4e-4 1.7e-4
Nicotine amide 1.2e-4 9.7e-4
Nicotinic acid 1.2e-4 9.6e-4
Biotin 9.5e-5 3.9e-4
Folic acid 9.5e-5 2.1e-4
Pyridoxamine hydrochloride 4.7e-5 2.3e-4
NaOH - -
Water - -
Vitamin K1 - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 50 ml Amino acid solution (filter sterilized)

78c CHOPPED MEAT MEDIUM FOR FRETIBACTERIUM SP.

Compound Conc. [g/L] Conc. [mM]
Ground beef 586.166 -
Casein peptone 35.17 -
Na-formate 7.034 103.43
Na-fumarate 7.034 43.953
K2HPO4 5.862 33.654
Yeast extract 5.862 -
Ethanol 2.227 48.349
L-Cysteine HCl x H2O 0.586 3.337
DL-Dithiothreitol 0.352 2.279
Haemin 0.003 0.004
Sodium resazurin 5.9e-4 0.002
Pyridoxine hydrochloride 1.2e-4 5.7e-4
Nicotinic acid 5.9e-5 4.8e-4
p-Aminobenzoic acid 5.9e-5 4.3e-4
(DL)-alpha-Lipoic acid 5.9e-5 2.8e-4
Thiamine HCl 5.9e-5 1.7e-4
Riboflavin 5.9e-5 1.6e-4
Calcium D-(+)-pantothenate 5.9e-5 -
Biotin 2.3e-5 9.6e-5
Folic acid 2.3e-5 5.3e-5
Vitamin B12 1.2e-6 8.6e-7
NaOH - -
Vitamin K1 - -
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Solutions:
  • 50 ml Clarified rumen fluid
  • 20 ml Fatty acid mixture
  • 1 ml Wolin's vitamin solution (10x)
  • 5 ml Haemin solution
  • 2 ml Vitamin K1 solution
Gas phase:
  • 80 % N2
  • 20 % CO2

78d MEDIUM FOR TREPONEMA PARVUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Glutathione 1.4 4.556
Na-pyruvate 0.5 4.544
L-Cysteine HCl 0.5 3.172
N-Acetylglucosamine 0.5 2.26
Ethanol 0.19 4.124
Casamino acids 0.16 -
Yeast extract 0.16 -
Tryptone peptone 0.16 -
Ascorbic acid 0.1 0.568
L-Glutamine 0.07 0.479
L-Histidin 0.06 0.387
L-Serin 0.05 0.476
Vitamin K3 0.05 -
Resazurin 1.0e-3 0.004
Hemin 5.0e-4 7.7e-4
p-Aminobenzoic acid 2.5e-4 0.002
alpha-lipoic acid 2.5e-4 0.001
Pantothenic acid 2.5e-4 0.001
Thiamine-HCl x 2 H2O 2.5e-4 6.7e-4
Riboflavin 2.5e-4 6.6e-4
Vitamin B12 2.5e-4 1.8e-4
Nicotine amide 1.3e-4 0.001
Nicotinic acid 1.3e-4 0.001
Biotin 1.0e-4 4.1e-4
Folic acid 1.0e-4 2.3e-4
Pyridoxamine hydrochloride 5.0e-5 2.4e-4
Calf serum - -
Vitamin K1 - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 5 ml Vitamin solution
  • 5 ml Amino acid solution
  • 5 ml Peptone mixture
  • 5 ml Hemin solution
  • 5 ml Ascorbic acid solution 2%(w/v)
  • 5 ml N-acetylglucosamin- and pyruvate solution
  • 10 ml Glutathione solution 14%(w/v)
  • 10 ml Sugar solution
Gas phase:
  • 80 % N2
  • 20 % CO2

104 PYG MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Peptone 5 -
Trypticase peptone 5 -
Beef extract 5 -
L-Cysteine HCl x H2O 0.5 -
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
Ethanol 0.004 0.082
Resazurin 1.0e-3 0.004
Tween 80 - -
Vitamin K1 - -
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution

110 CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
D-Glucose 4 22.203
Maltose 1 2.921
Cellobiose 1 2.921
Starch 1 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Sodium resazurin 5.0e-4 -
Water - -
Vitamin K1 - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
Gas phase:
  • 100 % N2

209 EUBACTERIUM LENTUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Arginine 5 28.703
Yeast extract 5 -
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Resazurin 1.0e-3 0.004
NaOH - -
Vitamin K1 - -
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Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
Sucrose 1.688 4.93
Na2CO3 1.488 14.04
Trypticase peptone 0.992 -
Ethanol 0.942 20.457
D-Glucose 0.794 4.405
D-Xylose 0.744 4.956
Propionic acid 0.591 7.977
Na-pyruvate 0.496 4.508
KH2PO4 0.496 3.645
NH4Cl 0.397 7.419
NaCl 0.397 6.79
DL-Dithiothreitol 0.397 2.572
Starch 0.397 -
MgCl2 x 6 H2O 0.327 1.61
L-Cysteine HCl x H2O 0.298 1.695
iso-Butyric acid 0.094 1.069
n-Valeric acid 0.093 0.912
iso-Valeric acid 0.092 0.904
CaCl2 x 2 H2O 0.05 0.337
HCl 0.002 0.068
FeCl2 x 4 H2O 0.001 0.007
NaOH 5.0e-4 0.012
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.3e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 9.9e-5 5.0e-4
Calcium pantothenate 9.9e-5 2.1e-4
Vitamin B12 9.9e-5 7.3e-5
p-Aminobenzoic acid 7.9e-5 5.8e-4
ZnCl2 6.9e-5 5.1e-4
Na2MoO4 x 2 H2O 3.6e-5 1.5e-4
NiCl2 x 6 H2O 2.4e-5 1.8e-4
D-(+)-biotin 2.0e-5 8.1e-5
H3BO3 6.0e-6 9.6e-5
Na2WO4 x 2 H2O 4.0e-6 1.2e-5
Na2SeO3 x 5 H2O 3.0e-6 1.1e-5
CuCl2 x 2 H2O 2.0e-6 1.2e-5
Distilled water - -
DL-2-Methylbutyric acid - -
Vitamin K1 - -
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

228 PEPTOCOCCUS GLYCINOPHILUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Trypticase peptone 5 -
Peptone 5 -
Beef extract 5 -
Glycine 3 39.963
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.02 0.081
CaCl2 x 2 H2O 0.01 0.068
Hemin 0.005 0.008
Ethanol 0.004 0.082
Resazurin 1.0e-3 0.004
NaOH - -
Vitamin K1 - -
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Solutions:
  • 40 ml Salt solution
  • 0.2 ml Vitamin K1 solution
  • 10 ml Haemin solution
Gas phase:
  • 80 % N2
  • 20 % CO2

328 PYG MEDIUM WITH VOLATILE FATTY ACIDS

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Beef extract 5 -
Trypticase 5 -
(NH4)2SO4 0.5 3.784
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.019 0.078
CaCl2 x 2 H2O 0.012 0.082
Haemin 0.005 0.008
Resazurin 1.0e-3 0.004
Vitamin K1 1.0e-3 0.002
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Solutions:
  • 40 ml Mineral solution
  • 3.1 ml Fatty acid mixture
Gas phase:
  • 100 % N2

413 BTU MEDIUM

Compound Conc. [g/L] Conc. [mM]
Ground beef 500 -
Casitone 30 -
Agar 15 44.599
K2HPO4 5 28.707
Yeast extract 5 -
Na-formiate 1.8 26.468
Na-fumarate 1.8 11.248
L-Cysteine HCl 0.5 3.172
Ethanol 0.19 4.124
Vitamin K3 0.05 -
Hemin 0.005 0.008
Resazurin 1.0e-3 0.004
Water - -
Vitamin K1 - -
Show more
Solutions:
  • 10 ml Haemin solution
  • 10 ml Vitamin K3 solution
  • 10 ml Vitamin K1 solution
  • 30 ml Formiate/Fumarate Solution
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