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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: ZnCl2

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28 PFENNIG'S MEDIUM I

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

28a THIORHODOCOCCUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaCl 20 342.231
NaHCO3 1.5 17.856
MgCl2 x 6 H2O 1 4.919
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Sodium Thiosulfate 0.5 3.162
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

28b THIORHODOCOCCUS MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaCl 15 256.674
MgCl2 x 6 H2O 2.5 12.297
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Na2S2O3 x 5 H2O 0.5 2.015
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
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Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)

29 PFENNIG'S MEDIUM II

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
Ammonium acetate 0.26 3.373
Pyruvic acid sodium salt 0.26 2.363
Dextrose 0.26 1.312
CaCl2 x 2 H2O 0.25 1.701
Yeast extract 0.25 -
Resazurin 0.002 0.01
HCl 0.002 0.055
FeSO4 x 7 H2O 0.002 0.006
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.1e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
MnCl2 x 4 H2O 1.0e-4 -
ZnCl2 7.3e-5 5.3e-4
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Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B

29a MEDIUM FOR CHLOROBIUM FERROOXIDANS

Compound Conc. [g/L] Conc. [mM]
FeSO4 1.519 10
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
Ammonium acetate 0.26 3.373
Pyruvic acid sodium salt 0.26 2.363
Dextrose 0.26 1.312
CaCl2 x 2 H2O 0.25 1.701
Yeast extract 0.25 -
Acetate 0.118 2
Resazurin 0.002 0.01
HCl 0.002 0.055
FeSO4 x 7 H2O 0.002 0.006
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.1e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
MnCl2 x 4 H2O 1.0e-4 5.3e-4
ZnCl2 7.3e-5 5.3e-4
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Solutions:
  • 1 ml Trace element solution SL-10 B
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F

39 PFENNIG'S MEDIUM I WITH YEAST EXTRACT

Compound Conc. [g/L] Conc. [mM]
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Yeast extract 0.5 -
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

40 PFENNIG'S MEDIUM II WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 1.5 17.856
MgSO4 x 7 H2O 0.52 2.11
Ammonium chloride 0.364 6.805
KCl 0.364 4.883
KH2PO4 0.364 2.675
Ammonium acetate 0.26 3.373
Pyruvic acid sodium salt 0.26 2.363
Dextrose 0.26 1.312
CaCl2 x 2 H2O 0.25 1.701
Yeast extract 0.25 -
Resazurin 0.002 0.01
HCl 0.002 0.055
FeSO4 x 7 H2O 0.002 0.006
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.1e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
MnCl2 x 4 H2O 1.0e-4 5.3e-4
ZnCl2 7.3e-5 5.3e-4
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Solution B
  • 50 ml Solution C
  • 450 ml Solution D
  • 26 ml Solution E
  • 10 ml Solution F
  • 1 ml Trace element solution SL-10 B

43 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 30 513.347
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

46 PFENNIG'S MEDIUM I WITH SALT

Compound Conc. [g/L] Conc. [mM]
NaCl 10 171.116
NaHCO3 1.5 17.856
Na2S x 9 H2O 0.593 2.467
MgSO4 x 7 H2O 0.5 2.029
Ammonium chloride 0.35 6.543
KCl 0.35 4.695
KH2PO4 0.35 2.572
Ammonium acetate 0.25 3.243
Pyruvic acid sodium salt 0.25 2.272
CaCl2 x 2 H2O 0.25 1.701
Dextrose 0.25 1.262
Yeast extract 0.25 -
Na2-EDTA 0.003 0.009
Resazurin 0.002 0.01
FeSO4 x 7 H2O 0.001 0.004
Vitamin B12 1.0e-3 7.4e-4
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 2 H2O 5.0e-5 -
ZnCl2 4.2e-5 3.1e-4
Show more
Solutions:
  • 460 ml Solution A
  • 40 ml Sulfide solution, 1.5%
  • 50 ml Bicarbonate solution
  • 450 ml Resazurin solution
  • 26 ml Pfennig's heterotrophic salts solution
  • 1 ml Vitamin B12 solution
  • 10 ml Neutralized sulfide solution 3% (w/v)
  • 1 ml Trace element solution SL-12 B

52 CLOSTRIDIUM KLUYVERI MEDIUM

Compound Conc. [g/L] Conc. [mM]
K-acetate 10 101.893
Na2CO3 1 9.435
Yeast extract 1 -
K2HPO4 0.31 1.78
NH4Cl 0.25 4.674
L-Cysteine HCl x H2O 0.25 1.423
Na2S x 9 H2O 0.25 1.041
KH2PO4 0.23 1.69
MgSO4 x 7 H2O 0.2 0.811
HCl 0.003 0.069
FeCl2 x 4 H2O 0.002 0.008
NaOH 5.0e-4 0.013
Sodium resazurin 5.0e-4 -
Pyridoxine hydrochloride 3.0e-4 0.001
Nicotinic acid 2.0e-4 0.002
Thiamine-HCl x 2 H2O 2.0e-4 5.4e-4
CoCl2 x 6 H2O 1.9e-4 0.001
MnCl2 x 4 H2O 1.0e-4 5.1e-4
Calcium pantothenate 1.0e-4 2.1e-4
Vitamin B12 1.0e-4 7.4e-5
p-Aminobenzoic acid 8.0e-5 5.8e-4
ZnCl2 7.0e-5 5.1e-4
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Solutions:
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2
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