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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: ZnSO4 x 7 H2O

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14 CLOSTRIDIUM FORMICACETICUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.837 -
K2HPO4 9.891 56.789
D-Fructose 4.946 27.451
Yeast extract 4.946 -
Na2CO3 0.989 9.332
Na-thioglycolate 0.742 6.502
Na2-EDTA 0.005 0.015
H3BO3 0.003 0.048
CoCl2 x 6 H2O 0.002 0.015
FeSO4 x 7 H2O 0.002 0.007
Pyridoxine hydrochloride 9.9e-4 0.005
ZnSO4 x 7 H2O 9.9e-4 0.003
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Solutions:
  • 10 ml Trace element solution SL-4
Gas phase:
  • 100 % N2

25 RHODOPSEUDOMONAS GLOBIFORMIS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Mannitol 1.5 8.234
KH2PO4 0.5 3.674
Na-gluconate 0.5 2.292
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
Yeast extract 0.25 -
Sodium thiosulfate pentahydrate 0.2 0.806
CaCl2 x 2 H2O 0.05 0.34
Fe(III) citrate 0.005 0.02
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
p-Aminobenzoic acid 1.0e-4 7.3e-4
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
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Solutions:
  • 1 ml Trace element solution SL-6

26 ACID RHODOSPIRILLACEAE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
L-Cysteine HCl 0.3 1.903
Yeast extract 0.2 -
CaCl2 x 2 H2O 0.05 0.34
Resazurin 0.005 0.022
Fe(III) citrate 0.005 0.02
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
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Solutions:
  • 1 ml Trace element solution SL-6
Gas phase:
  • 100 % N2

27 RHODOSPIRILLACEAE MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Disodium succinate 1 6.171
Ammonium acetate 0.5 6.487
KH2PO4 0.5 3.674
NH4Cl 0.4 7.478
NaCl 0.4 6.845
MgSO4 x 7 H2O 0.4 1.623
L-Cysteine HCl 0.3 1.903
Yeast extract 0.3 -
CaCl2 x 2 H2O 0.05 0.34
Resazurin 0.005 0.022
Fe(III) citrate 0.005 -
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 0.002
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
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Solutions:
  • 1 ml Trace element solution SL-6

55 FRUCTOSE MINERAL MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 15 44.599
Na2HPO4 x 12 H2O 9 63.399
Fructose 5 27.753
KH2PO4 1.5 11.023
NH4Cl 1 18.695
MgSO4 x 7 H2O 0.2 0.811
CaCl2 x 2 H2O 0.01 0.068
Ferric ammonium citrate 0.005 0.019
H3BO3 9.0e-5 0.001
CoCl2 x 6 H2O 6.0e-5 4.6e-4
ZnSO4 x 7 H2O 3.0e-5 1.0e-4
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Solutions:
  • 500 ml Solution A
  • 300 ml Solution B
  • 200 ml Solution C
  • 0.3 ml Trace element solution SL-6

68 HALOTHIOBACILLUS NEAPOLITANUS MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		15 44.599
Na2S2O3 x 5 H2O 10 40.292
KH2PO4 4 29.393
K2HPO4 4 22.965
MgSO4 x 7 H2O 0.8 3.246
NH4Cl 0.4 7.478
Na2-EDTA 0.25 0.744
ZnSO4 x 7 H2O 0.11 0.383
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Solutions:
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

69 STARKEYA NOVELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar
optional 		14.892 -
Na2HPO4 x 12 H2O 10.524 74.133
Na2S2O3 x 5 H2O 4.975 20.046
KH2PO4 1.489 10.943
NH4Cl 0.298 5.568
Yeast extract 0.298 -
Na2-EDTA 0.249 0.74
ZnSO4 x 7 H2O 0.109 0.381
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Solutions:
  • 900 ml Solution A
  • 50 ml Solution B
  • 5 ml Solution C
  • 50 ml Solution D
  • 5 ml Trace element solution (Vishniac & Santer, 1957)

81 MINERAL MEDIUM FOR CHEMOLITHOTROPHIC GROWTH (H-3)

Compound Conc. [g/L] Conc. [mM]
Agar 20.101 59.764
Na2HPO4 x 2 H2O 2.915 20.531
KH2PO4 2.312 16.986
NH4Cl 1.005 18.789
MgSO4 x 7 H2O 0.503 2.039
Ferric ammonium citrate 0.05 0.192
CaCl2 x 2 H2O 0.01 0.068
NaVO3 x H2O 0.005 0.036
Nicotinic acid 0.003 0.02
Pyridoxine hydrochloride 0.003 0.012
Calcium pantothenate 0.003 0.005
Thiamine-HCl x 2 H2O 0.003 -
H3BO3 0.002 0.024
CoCl2 x 6 H2O 0.001 0.008
ZnSO4 x 7 H2O 5.0e-4 0.002
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Solutions:
  • 5 ml Standard vitamin solution
  • 50 ml Solution A
  • 920 ml Solution B
  • 20 ml Solution C
  • 5 ml Trace element solution SL-6

81a THIOMONAS ISLANDICA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2HPO4 x 2 H2O 2.868 20.206
K2HPO4 2.275 13.061
NH4Cl 0.989 18.492
NaHCO3 0.495 5.887
MgSO4 x 7 H2O 0.495 2.007
Ferric ammonium citrate 0.049 -
CaCl2 x 2 H2O 0.01 0.067
NaVO3 x H2O 0.005 0.035
H3BO3 0.001 0.024
CoCl2 x 6 H2O 9.9e-4 0.008
ZnSO4 x 7 H2O 4.9e-4 0.002
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Solutions:
  • 5 ml Trace element solution SL-6
  • 1 ml Seven vitamins solution
Gas phase:
  • 53 % H2
  • 27 % N2
  • 20 % CO2

84 ROLLED OATS MINERAL MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 20 -
Rolled oats 20 -
MnCl2 x 4 H2O 1.0e-3 0.005
FeSO4 x 7 H2O 1.0e-3 0.004
ZnSO4 x 7 H2O 1.0e-3 0.003
Distilled water - -
Solutions:
  • 1 ml Trace element solution
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