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The medium composition is currently in its beta status, meaning that some concentrations are not correct and need further adjustments. Keep this in mind while trying this out.

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Current criteria: iso-Valeric acid

Your search received 13 results.

212a TREPONEMA RECTALE MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2SO4 2.778 19.556
Trehalose 1.884 5.504
Maltose x H2O 1.786 5.217
Acetic acid 1.763 29.355
Cellobiose 1.688 4.93
Sucrose 1.688 4.93
Na2CO3 1.488 14.04
Trypticase peptone 0.992 -
Ethanol 0.942 20.457
D-Glucose 0.794 4.405
D-Xylose 0.744 4.956
Propionic acid 0.591 7.977
Na-pyruvate 0.496 4.508
KH2PO4 0.496 3.645
NH4Cl 0.397 7.419
NaCl 0.397 6.79
DL-Dithiothreitol 0.397 2.572
Starch 0.397 -
MgCl2 x 6 H2O 0.327 1.61
L-Cysteine HCl x H2O 0.298 1.695
iso-Butyric acid 0.094 1.069
n-Valeric acid 0.093 0.912
iso-Valeric acid 0.092 0.904
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Solutions:
  • 917 ml Solution A
  • 30 ml Solution B
  • 4 ml Solution C
  • 4 ml Solution D
  • 30 ml Solution E
  • 1 ml Solution F
  • 2 ml Solution G
  • 10 ml Solution H
  • 10 ml Solution I
  • 1 ml Trace element solution SL-10
  • 1 ml Selenite-tungstate solution
  • 50 ml Clarified rumen fluid
  • 1 ml Vitamin K1 solution
  • 3.1 ml Volatile fatty acid mixture
  • 30 ml Sugar mix
  • 1 ml Seven vitamins solution
Gas phase:
  • 80 % N2
  • 20 % CO2

323 TREPONEMA SACCHAROPHILUM MEDIUM

Compound Conc. [g/L] Conc. [mM]
Agar 12 35.679
Na2CO3 2.5 23.588
D-Glucose 2 11.101
Trypticase peptone 2 -
Yeast extract 2 -
L-Cysteine HCl x H2O 1 5.694
NaCl 0.9 15.4
(NH4)2SO4 0.9 6.811
KH2PO4 0.45 3.307
K2HPO4 0.45 2.584
MgSO4 x 7 H2O 0.19 0.771
CaCl2 x 2 H2O 0.12 0.816
Sodium resazurin 5.0e-4 -
n-Butyric acid - -
iso-Butyric acid - -
DL-2-Methylbutyric acid - -
n-Valeric acid - -
Distilled water - -
iso-Valeric acid - -
Gas phase:
  • 100 % CO2

328 PYG MEDIUM WITH VOLATILE FATTY ACIDS

Compound Conc. [g/L] Conc. [mM]
Yeast extract 10 -
Glucose 5 27.753
Beef extract 5 -
Trypticase 5 -
(NH4)2SO4 0.5 3.784
L-Cysteine HCl x H2O 0.5 2.847
NaHCO3 0.4 4.762
NaCl 0.08 1.369
KH2PO4 0.04 0.294
K2HPO4 0.04 0.23
MgSO4 x 7 H2O 0.019 0.078
CaCl2 x 2 H2O 0.012 0.082
Haemin 0.005 0.008
Resazurin 1.0e-3 0.004
Vitamin K1 1.0e-3 0.002
Distilled water - -
iso-Valeric acid - -
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Solutions:
  • 40 ml Mineral solution
  • 3.1 ml Fatty acid mixture
Gas phase:
  • 100 % N2

704 BUTYRIVIBRIO SP. MEDIUM

Compound Conc. [g/L] Conc. [mM]
Na2CO3 3.996 37.702
Yeast extract 1.998 -
Trypticase peptone 1.998 -
D-Glucose 0.999 5.545
Cellobiose 0.999 2.919
Maltose 0.999 2.919
Starch 0.999 -
NaCl 0.899 15.385
Glycerol 0.5 5.424
(NH4)2SO4 0.45 3.402
KH2PO4 0.45 3.303
K2HPO4 0.3 1.721
L-Cysteine HCl x H2O 0.25 1.422
Na2S x 9 H2O 0.25 1.04
MgSO4 x 7 H2O 0.187 0.76
CaCl2 x 2 H2O 0.12 0.815
Haemin 1.0e-3 0.002
Sodium resazurin 5.0e-4 0.002
Propionic acid - -
NaOH - -
iso-Valeric acid - -
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Solutions:
  • 75 ml Mineral solution
  • 150 ml Clarified rumen fluid
  • 3.1 ml Volatile fatty acid mixture
  • 2 ml Haemin solution
Gas phase:
  • 100 % CO2

1006 ALLISONELLA MEDIUM

Compound Conc. [g/L] Conc. [mM]
DL-Histidine 7.769 50.072
Na2CO3 3.984 37.59
Yeast extract 3.984 -
Trypticase peptone 0.996 -
L-Cysteine HCl x H2O 0.498 2.835
NaCl 0.478 8.181
(NH4)2SO4 0.478 3.618
KH2PO4 0.289 2.123
K2HPO4 0.289 1.658
MgSO4 x 7 H2O 0.1 0.404
CaCl2 x 2 H2O 0.064 0.434
Sodium resazurin 5.0e-4 0.002
iso-Valeric acid - -
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Solutions:
  • 3.1 ml Volatile fatty acid mixture
Gas phase:
  • 100 % CO2

1455 ALTERNATIVE RUMEN BACTERIA MEDIUM

Compound Conc. [g/L] Conc. [mM]
NaHCO3 6.362 75.73
Brain heart infusion 5.964 -
Disodium succinate 4.97 30.671
Yeast extract 4.97 -
NaCl 0.447 7.654
(NH4)2SO4 0.447 3.385
L-Cysteine HCl x H2O 0.298 1.698
Na2S x 9 H2O 0.298 1.242
KH2PO4 0.229 1.68
K2HPO4 0.229 1.313
MgSO4 x 7 H2O 0.089 0.363
CaCl2 x 2 H2O 0.06 0.406
Haemin 9.9e-4 0.002
Sodium resazurin 5.0e-4 0.002
Pyridoxine hydrochloride 9.9e-5 4.8e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.1e-5
Folic acid 2.0e-5 4.5e-5
Vitamin B12 9.9e-7 7.3e-7
iso-Butyric acid - -
Distilled water - -
NaOH - -
iso-Valeric acid - -
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Solutions:
  • 3.1 ml Volatile fatty acid mixture
  • 2 ml Haemin solution
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 100 % CO2

1611 YCFA MEDIUM (modified)

Compound Conc. [g/L] Conc. [mM]
Casitone 10 -
Glucose 5 27.753
NaHCO3 4 47.615
Yeast extract 2.5 -
Acetic acid 1.787 29.758
L-Cysteine HCl 1 6.344
NaCl 0.9 15.4
Propionic acid 0.626 8.445
KH2PO4 0.45 3.307
K2HPO4 0.45 2.584
CaCl2 x 2 H2O 0.09 0.612
n-Valeric acid 0.085 0.827
iso-Valeric acid 0.084 0.82
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Solutions:
  • 2.7 ml Volatile fatty acids
  • 0.1 ml Vitamin solution
  • 10 ml Main sol. 1611

J133 MEDIUM 10

Compound Conc. [g/L] Conc. [mM]
Agar 17.192 51.116
Trypticase peptone 1.91 -
Glucose 0.478 2.651
Cellobiose 0.478 1.395
Yeast extract 0.478 -
Starch 0.478 -
(NH4)2SO4 0.43 3.253
NaCl 0.423 7.232
K2HPO4 0.279 1.604
KH2PO4 0.168 1.237
MgSO4 x H2O 0.09 0.744
CaCl2 0.043 0.387
DL-alpha-Methyl butyric acid - -
L-Ascorbic acid - -
L-Cysteine HCl x H2O - -
Toray silicone SH 5535 - -
Resazurin - -
Hemin - -
Acetic acid - -
Propionic acid - -
Distilled water - -
n-Butyric acid - -
iso-Butyric acid - -
n-Valeric acid - -
iso-Valeric acid - -
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Solutions:
  • 3.1 ml VFA solution
  • 37.5 ml Salt solution No. 1
  • 37.5 ml Salt solution No. 2

J705 SELENOMONAS WG MEDIUM

Compound Conc. [g/L] Conc. [mM]
Tryptone 4.902 68.964
Glucose 1.961 10.884
Yeast extract 1.961 -
NaCl 0.98 16.776
(NH4)2SO4 0.98 7.419
KH2PO4 0.49 3.602
K2HPO4 0.49 2.814
CaCl2 x 2 H2O 0.098 0.667
MgSO4 x 7 H2O 0.098 0.398
Resazurin 9.8e-4 0.004
L-Cysteine HCl x H2O - -
Distilled water - -
n-Valeric acid - -
iso-Valeric acid - -
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Solutions:
  • 10 ml VFA solution

J1000 PYVG MEDIUM

Compound Conc. [g/L] Conc. [mM]
Glucose 9.399 52.168
Yeast extract 9.399 -
Trypticase peptone 4.699 -
Bacto peptone 4.699 -
L-Cysteine HCl x H2O 0.47 -
NaHCO3 0.376 4.475
NaCl 0.075 1.287
KH2PO4 0.038 0.276
K2HPO4 0.038 0.216
MgSO4 x 7 H2O 0.018 0.073
CaCl2 x 2 H2O 0.008 0.051
Distilled water - -
Acetic acid - -
DL-alpha-Methyl butyric acid - -
Resazurin - -
n-Valeric acid - -
iso-Butyric acid - -
n-Butyric acid - -
Propionic acid - -
iso-Valeric acid - -
Solutions:
  • 10 ml Hemin solution
  • 10 ml Hemin solution
  • 3.1 ml VFA solution
  • 40 ml Salt solution
Gas phase:
  • 100 % N2
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