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1074 DESULFOGLAEBA MEDIUM

Compound Conc. [g/L] Conc. [mM]
Sea Salt 24.925 -
Na2SO4 3.49 24.567
Na2CO3 1.496 14.11
Na-pyruvate 0.997 9.06
NH4Cl 0.249 4.66
KH2PO4 0.199 1.465
L-Cysteine HCl x H2O 0.1 0.568
Na2S x 9 H2O 0.1 0.415
Yeast extract 0.05 -
MgSO4 x 7 H2O 0.03 0.121
NaCl 0.01 0.171
MnSO4 x H2O 0.005 0.029
(NH4)2Ni(SO4)2 x 6 H2O 0.003 0.007
CoCl2 x 6 H2O 0.002 0.014
ZnSO4 x 7 H2O 0.002 0.006
CaCl2 x 2 H2O 1.0e-3 0.007
FeSO4 x 7 H2O 1.0e-3 0.004
Sodium resazurin 5.0e-4 0.002
AlK(SO4)2 x 12 H2O 1.8e-4 3.8e-4
H3BO3 1.0e-4 0.002
Na2SeO4 1.0e-4 5.3e-4
Pyridoxine hydrochloride 1.0e-4 4.8e-4
Na2MoO4 x 2 H2O 1.0e-4 4.1e-4
CuSO4 x 5 H2O 1.0e-4 4.0e-4
Na2WO4 x 2 H2O 1.0e-4 3.0e-4
Nicotinic acid 5.0e-5 4.0e-4
p-Aminobenzoic acid 5.0e-5 3.6e-4
(DL)-alpha-Lipoic acid 5.0e-5 2.4e-4
Calcium D-(+)-pantothenate 5.0e-5 2.1e-4
Thiamine HCl 5.0e-5 1.5e-4
Riboflavin 5.0e-5 1.3e-4
Biotin 2.0e-5 8.2e-5
Folic acid 2.0e-5 4.5e-5
Vitamin B12 1.0e-6 7.4e-7
Distilled water - -
n-Decane - -
Solutions:
  • 1 ml Wolfe's mineral elixir
  • 1 ml Wolin's vitamin solution (10x)
Gas phase:
  • 80 % N2
  • 20 % CO2