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1. NUTRIENT AGAR

Culture medium recipe

Vol.
×

M 1 #

ml
Solution occurs in 12 other media (first defined in medium 1 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5 g 5 -
2 3 g 3 -
3
optional
15 g 15 -
4 1000 ml 998.207 55408.8
5 , recommended for sporulation of Bacillus strains
optional
10 mg 0.01 0.059
Instructions:
  1. Adjust pH to 7.0. For Bacillus strains the addition of 10.0 mg MnSO4 x H2O is recommended for sporulation.

Metadata

Media status: Official DSMZ medium
Synonyms: Nutrient Broth, NB Medium
Source: DSMZ
Taxonomic range:  Bacteria
Taxonomic range:  Phages
Medium type: Complex medium
Final pH: 7.0

Associated strains

All strains for this medium: 2098
Strains with modifications: 216
1 NUTRIENT AGAR
a REACTIVATION WITH LIQUID MEDIUM 1
b GLUTAMINE MEDIUM

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
Agar
optional
15 -
Peptone 5 -
Meat extract 3 -
MnSO4 x H2O
optional
0.01 0.059

Cultivation metadata from 2098 strains BacDive

halophily not 112 strains
halophily slightly 55 strains
halophily moderate 41 strains
halophily extreme 1 strain
pH 6 - 8 140 strains
pH 4 - 6 130 strains
pH 8 - 10 122 strains
pH 10 - 14 37 strains
pH 2 - 4 16 strains
phyla 7 2040 strains
nutrition chemoorganotroph 11 strains
nutrition chemolithoautotroph 3 strains
nutrition organotroph 1 strain
nutrition chemolithotroph 1 strain
nutrition chemoheterotroph 1 strain
oxygen aerobe 311 strains
oxygen facultative anaerobe 43 strains
oxygen anaerobe 10 strains
oxygen obligate aerobe 10 strains
oxygen microaerophile 7 strains
oxygen facultative aerobe 4 strains
temperature mesophilic 1928 strains
temperature thermophilic 130 strains
temperature psychrophilic 71 strains
temperature hyperthermophilic 3 strains

Similar media

1a. REACTIVATION WITH LIQUID MEDIUM 1 100 %
302. NUTRIENT BROTH WITH 10% HORSE SERUM 96 %
238. STREPTOMYCIN NUTRIENT AGAR 96 %
118. DAP-NUTRIENT AGAR 96 %
1b. GLUTAMINE MEDIUM 92 %

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