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1002. ECTOTHIORHODOSYNUS MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.5 g 0.5 3.671
2 0.5 g 0.5 9.338
3 20.0 g 19.98 341.875
4 0.2 g 0.2 0.983
5 0.1 g 0.1 0.9
6 5.0 g 4.995 47.128
7 5.0 g 4.995 59.46
8 1.0 g 0.999 12.178
9 0.1 g 0.1 -
10 0.5 g 0.5 2.013
11 0.1 g 0.1 0.416
12 Trace element solution SL-6 1.0 ml - -
13 20.0 μg 2.0e-5 1.3e-5
14 1000.0 ml 997.21 55353.5
Instructions:
  1. Prepare the NH4Cl, CaCl2, carbonate, bicarbonate and sulfide as a single sterile stock solution and add to the sterile medium after autoclaving. The pH should be adjust to 9-9.5.
Solution occurs in 38 other media (first defined in medium 1142 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 0.10 g 0.1 0.348
2 0.03 g 0.03 0.152
3 0.30 g 0.3 4.852
4 0.20 g 0.2 0.841
5 0.01 g 0.01 0.059
6 0.02 g 0.02 0.084
7 0.03 g 0.03 0.124
8 1000.00 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 9.2

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
NaCl 19.98 341.875
NaHCO3 4.995 59.46
Na2CO3 4.995 47.128
Sodium acetate 0.999 12.178
NH4Cl 0.5 9.338
KH2PO4 0.5 3.671
Na2S2O3 x 5 H2O 0.5 2.013
MgCl2 x 6 H2O 0.2 0.983
CaCl2 0.1 0.9
Na2S x 9 H2O 0.1 0.416
Yeast extract 0.1 -
H3BO3 3.0e-4 0.005
CoCl2 x 6 H2O 2.0e-4 8.4e-4
ZnSO4 x 7 H2O 1.0e-4 3.5e-4
MnCl2 x 4 H2O 3.0e-5 1.5e-4
Na2MoO4 x 2 H2O 3.0e-5 1.2e-4
NiCl2 x 6 H2O 2.0e-5 8.4e-5
Vitamin B12 2.0e-5 1.3e-5
CuCl2 x 2 H2O 1.0e-5 5.9e-5

Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
temperature mesophilic 1 strain

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