This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Water | 1000.00 | ml | - | - | |
1 Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds: | |||||
MgSO4 x 7 H2O | 7.00 | g | 7 | 28.4 | |
NaS2O3 | 2.00 | g | 2 | - | |
CaCl2 x 2 H2O | 0.40 | g | 0.4 | 2.721 | |
KCl | 0.48 | g | 0.48 | 6.439 | |
MgCl2 | 0.78 | g | 0.78 | 8.192 | |
MES | 1.95 | g | 1.95 | 9.988 | |
2 Add solutions A, B, and Trace mineral solution (see recipes below): | |||||
Solution A (100x solution) | 2.00 | ml | - | - | |
Solution B (500x solution) | 1.50 | ml | - | - | |
Trace mineral solution (100x) | 10.00 | ml | - | - | |
3 pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121°C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP). |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
NH4Cl | 100 | g | 100 | 1869.51 | |
MgCl2 x H2O | 100 | g | 100 | 883.189 | |
CaCl2 x 2 H2O | 40 | g | 40 | 272.081 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust the pH to 4 with HCl. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
K2HPO4 x 3 H2O | 200 | g | 200 | 1148.26 |
Distilled water | 1000 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Na-EDTA x 2 H2O | 500 | mg | 0.5 | 1.487 | |
CoCl2 x 6 H2O | 150 | mg | 0.15 | 1.155 | |
MnCl2 x 4 H2O | 100 | mg | 0.1 | 0.505 | |
FeSO4 x 7 H2O | 100 | mg | 0.1 | 0.36 | |
ZnCl2 | 100 | mg | 0.1 | 0.734 | |
AlCl3 x 6 H2O | 40 | mg | 0.04 | 0.166 | |
Na-tungstate x 2 H2O | 30 | mg | 0.03 | - | |
CuCl | 20 | mg | 0.02 | 0.202 | |
NiSO4 x 6 H2O | 20 | mg | 0.02 | 0.129 | |
Se-acid | 10 | mg | 0.01 | 0.069 | |
HBO3 | 10 | mg | 0.01 | 0.167 | |
Na2MoO4 x 2 H2O | 10 | mg | 0.01 | 0.041 | |
Distilled water | 1000 | ml | - | - | |
1 Adjust the pH of the solution to 3.0 with HCl. |
Last modified: | 15.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Defined medium |
Final pH: | n.d. |
Equipment needed: | Anaerobic workbench, Autoclave |
Edit in Medium builder |
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
MgSO4 x 7 H2O | 7 | 28.4 |
NaS2O3 | 2 | - |
MES | 1.95 | 9.988 |
MgCl2 | 0.78 | 8.192 |
KCl | 0.48 | 6.439 |
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