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1003. HYDROGEN-OXYDIZING MEDIUM

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Anaerobic workbench
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1000.00 - -
2 7.00 g 7 28.4
3 2.00 g 2 -
4 0.40 g 0.4 2.721
5 0.48 g 0.48 6.439
6 0.78 g 0.78 8.192
7 1.95 g 1.95 9.988
8 Solution A (100x solution) 2.00 ml - -
9 Solution B (500x solution) 1.50 ml - -
10 Trace mineral solution (100x) 10.00 ml - -
Instructions:
  1. Place 1000 ml of anaerobic water (CO2 water) in a flask. Add the following compounds: #2-7
  2. Add solutions A, B, and D (see recipes below):
  3. pH the media to 6.0 and gas the flask containing the media with CO2 for 20 min at least. Gas first culture tube in rack and place 5 ml of media in previous tube. Place stopper on loaded culture tube, crimp tube cap onto stopper. Autoclave the medium for 20 min., 121°C. Add 1 ml of O2 to each tube before inoculation. After the inoculation the tubes are pressurized with H2 (138 KP).
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 100 g 100 1869.47
2 100 g 100 883.189
3 40 g 40 272.081
4 1000 ml 998.207 55408.8
5 , required for pH adjustment x - -
Instructions:
  1. Adjust the pH to 4 with HCl.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 200 g 200 876.347
2 1000 ml 998.207 55408.8
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 500 mg 0.5 1.343
2 150 mg 0.15 0.63
3 100 mg 0.1 0.505
4 100 mg 0.1 0.36
5 100 mg 0.1 0.734
6 40 mg 0.04 0.166
7 30 mg 0.03 0.091
8 20 mg 0.02 0.202
9 20 mg 0.02 0.076
10 10 mg 0.01 0.069
11 10 mg 0.01 0.167
12 10 mg 0.01 0.041
13 1000 ml 998.207 55408.8
14 , required for pH adjustment x - -
Instructions:
  1. Adjust the pH of the solution to 3.0 with HCl.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 4.0
Equipment needed: Anaerobic workbench, Autoclave

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 13.476 748.019
MgSO4 x 7 H2O 7 28.4
NaS2O3 2 -
2-(N-Morpholino)ethanesulfonic acid 1.95 9.988
MgCl2 0.78 8.192
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
phyla 1 1 strain
temperature thermophilic 1 strain

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