1006
1006 .
ALLISONELLA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1006 #
ml
Equipment needed: Autoclave
Hungate tubes
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| DL-Histidine | 7.80 | g | 7.769 | 50.072 | |
| Yeast extract | 4.00 | g | 3.984 | - | |
|
Trypticase peptone
(BD BBL) |
1.00 | g | 0.996 | - | |
| K2HPO4 | 0.29 | g | 0.289 | 1.658 | |
| KH2PO4 | 0.29 | g | 0.289 | 2.123 | |
| (NH4)2SO4 | 0.48 | g | 0.478 | 3.618 | |
| NaCl | 0.48 | g | 0.478 | 8.181 | |
| MgSO4 x 7 H2O | 0.10 | g | 0.1 | 0.404 | |
| CaCl2 x 2 H2O | 64.00 | mg | 0.064 | 0.434 | |
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
| Na2CO3 | 4.00 | g | 3.984 | 37.59 | |
| Volatile fatty acid mixture | 3.10 | ml | - | - | |
| L-Cysteine HCl x H2O | 0.50 | g | 0.498 | 2.835 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary. | |||||
Solution occurs in 4
other media (first defined in Medium 330).
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Acetic acid | 548.50 | ml | 573.182 | 9545.09 |
| Propionic acid | 193.50 | ml | 192.145 | 2593.76 |
| Butyric acid | 129.00 | ml | - | - |
| n-Valeric acid | 32.25 | ml | 30.283 | 296.512 |
| iso-Butyric acid | 32.25 | ml | 30.638 | 347.719 |
| DL-2-Methylbutyric acid | 32.25 | ml | - | - |
| iso-Valeric acid | 32.25 | ml | 30.025 | 293.986 |
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 6.0 |
| Equipment needed: | Autoclave, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % CO2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 1 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| DL-Histidine | 7.769 | 50.072 |
| Na2CO3 | 3.984 | 37.59 |
| Yeast extract | 3.984 | - |
| Trypticase peptone | 0.996 | - |
| L-Cysteine HCl x H2O | 0.498 | 2.835 |
| Show more | ||
