DSMZ 1006 . ALLISONELLA MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Autoclave Hungate tubes Gassing station
Compound Amount Unit Conc. [g/L] Conc. [mM]
DL-Histidine 7.80 g 7.769 50.072
Yeast extract 4.00 g 3.984 -
Trypticase peptone
(BD BBL)
1.00 g 0.996 -
K2HPO4 0.29 g 0.289 1.658
KH2PO4 0.29 g 0.289 2.123
(NH4)2SO4 0.48 g 0.478 3.618
NaCl 0.48 g 0.478 8.181
MgSO4 x 7 H2O 0.10 g 0.1 0.404
CaCl2 x 2 H2O 64.00 mg 0.064 0.434
Sodium resazurin
(0.1% w/v)
0.50 ml 5.0e-4 0.002
Na2CO3 4.00 g 3.984 37.59
Volatile fatty acid mixture 3.10 ml - -
L-Cysteine HCl x H2O 0.50 g 0.498 2.835
Distilled water 1000.00 ml - -
1 Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary.
Solution occurs in 4 other media (first defined in Medium 330).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Acetic acid 548.50 ml - -
Propionic acid 193.50 ml - -
Butyric acid 129.00 ml - -
n-Valeric acid 32.25 ml - -
iso-Butyric acid 32.25 ml - -
DL-2-Methylbutyric acid 32.25 ml - -
iso-Valeric acid 32.25 ml - -

Metadata

Last modified: 11.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 6.0
Equipment needed: Autoclave, Gassing station, Hungate tubes
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Gas composition

  • 100 % CO2

Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .

Associated strains

All strains for this medium: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
DL-Histidine 7.769 50.072
Na2CO3 3.984 37.59
Yeast extract 3.984 -
Trypticase peptone 0.996 -
L-Cysteine HCl x H2O 0.498 2.835
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen anaerobe 1 strain
temperature mesophilic 1 strain