Compound | Amount | Unit | |||
---|---|---|---|---|---|
|
DL-Histidine | 7.80 | g | ||
|
Yeast extract | 4.00 | g | ||
|
Trypticase peptone
(BD BBL) |
1.00 | g | ||
|
K2HPO4 | 0.29 | g | ||
|
KH2PO4 | 0.29 | g | ||
|
(NH4)2SO4 | 0.48 | g | ||
|
NaCl | 0.48 | g | ||
|
MgSO4 x 7 H2O | 0.10 | g | ||
|
CaCl2 x 2 H2O | 64.00 | mg | ||
|
Sodium resazurin
(0.1% w/v) |
0.50 | ml | ||
|
Na2CO3 | 4.00 | g | ||
|
Volatile fatty acid mixture | 3.10 | ml | ||
|
L-Cysteine HCl x H2O | 0.50 | g | ||
|
Distilled water | 1000.00 | ml | ||
1 Dissolve ingredients (except carbonate, fatty acid mixture and cysteine), bring medium to the boil, then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate, fatty acid mixture and cysteine and adjust pH to 6.0. Dispense medium under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Adjust pH of complete medium to 6.0, if necessary. |
Compound | Amount | Unit | |
---|---|---|---|
|
Acetic acid | 548.50 | ml |
|
Propionic acid | 193.50 | ml |
|
Butyric acid | 129.00 | ml |
|
n-Valeric acid | 32.25 | ml |
|
iso-Butyric acid | 32.25 | ml |
|
DL-2-Methylbutyric acid | 32.25 | ml |
|
iso-Valeric acid | 32.25 | ml |