1008
1008 .
SEDIMINISPIROCHAETA MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Medium for Sediminispirochaeta bajacaliforniensis DSM 16054
This strain uses the standard medium without modifications.
Growth conditions: 3-7 days
anaerobic
30 °C
Main sol. 1008 #
ml
Equipment needed: Hungate tubes
Filter
Autoclave
Gassing station
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Trypticase peptone
(BD BBL) |
2.0 | g | 2 | - | |
| Yeast extract | 1.0 | g | 1 | - | |
|
Sodium resazurin
(0.1% w/v) |
0.5 | ml | 5.0e-4 | - | |
| Na-thioglycolate | 1.0 | g | 1 | 8.764 | |
| Cellobiose | 2.0 | g | 2 | 5.843 | |
| Charcoal-filtered, natural seawater | 800.0 | ml | - | - | |
| Distilled water | 200.0 | ml | - | - | |
| 1 Dissolve ingredients except thioglycolate and cellobiose, then sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add thioglycolate and adjust pH of medium to 7.5 with 10 N KOH. Dispense medium under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave at 121°C for 15 min. Add cellobiose from a sterile anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. | |||||
| 2 Note: Bottled seawater from Biomaris GmbH can be used instead of filtered seawater. | |||||
Metadata
| Last modified: | 11.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.5 |
| Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
| Edit in Medium builder | |
Gas composition
- 100 % N2
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
Associated strains
| All strains for this medium: | 5 |
| Strains with modifications: | 2 |
Sediminispirochaeta bajacaliforniensis DSM 16054
No modifications necessary for this strain.
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Cellobiose | 2 | 5.843 |
| Trypticase peptone | 2 | - |
| Na-thioglycolate | 1 | 8.764 |
| Yeast extract | 1 | - |
| Sodium resazurin | 5.0e-4 | - |
| Show more | ||
