Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
KCl | 0.33 | g | 0.329 | 4.418 | |
MgSO4 x 7 H2O | 3.45 | g | 3.443 | 13.969 | |
MgCl2 x 6 H2O | 2.75 | g | 2.745 | 13.5 | |
NaCl | 20.00 | g | 19.96 | 341.548 | |
CaCl2 x 2 H2O | 0.15 | g | 0.15 | 1.018 | |
NH4Cl | 0.25 | g | 0.25 | 4.664 | |
K2HPO4 | 0.15 | g | 0.15 | 0.859 | |
Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g | 0.01 | - | |
Yeast extract | 0.10 | g | 0.1 | - | |
Trace element solution SL-6 | 1.00 | ml | - | - | |
Sodium resazurin
(0.1% w/v) |
0.50 | ml | 5.0e-4 | 0.002 | |
Na2CO3 | 1.00 | g | 0.998 | 9.416 | |
D-Glucose | 2.00 | g | 1.996 | 11.079 | |
Wolin's vitamin solution (10x) | 1.00 | ml | - | - | |
L-Cysteine HCl x H2O | 0.30 | g | 0.299 | 1.705 | |
Na2S x 9 H2O | 0.30 | g | 0.299 | 1.247 | |
Distilled water | 1000.00 | ml | - | - | |
1 Dissolve ingredients except carbonate, glucose, vitamins, cysteine and sulfide, then sparge medium with 80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add vitamins, glucose, cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas (vitamins are sterilized by filtration) and carbonate from a sterile anoxic stock solution prepared under 80% N2 and 20% CO2 gas mixture. Adjust pH of complete medium to 6.0 - 6.5, if necessary. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
ZnSO4 x 7 H2O | 0.10 | g | 0.1 | 0.348 |
MnCl2 x 4 H2O | 0.03 | g | 0.03 | 0.152 |
H3BO3 | 0.30 | g | 0.3 | 4.851 |
CoCl2 x 6 H2O | 0.20 | g | 0.2 | 1.54 |
CuCl2 x 2 H2O | 0.01 | g | 0.01 | 0.059 |
NiCl2 x 6 H2O | 0.02 | g | 0.02 | 0.154 |
Na2MoO4 x 2 H2O | 0.03 | g | 0.03 | 0.124 |
Distilled water | 1000.00 | ml | - | - |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 20 | mg | 0.02 | 0.082 |
Folic acid | 20 | mg | 0.02 | 0.045 |
Pyridoxine hydrochloride | 100 | mg | 0.1 | 0.486 |
Thiamine HCl | 50 | mg | 0.05 | 0.148 |
Riboflavin | 50 | mg | 0.05 | 0.133 |
Nicotinic acid | 50 | mg | 0.05 | 0.406 |
Calcium D-(+)-pantothenate | 50 | mg | 0.05 | 0.21 |
Vitamin B12 | 1 | mg | 1.0e-3 | 7.4e-4 |
p-Aminobenzoic acid | 50 | mg | 0.05 | 0.365 |
(DL)-alpha-Lipoic acid | 50 | mg | 0.05 | 0.242 |
Distilled water | 1000 | ml | - | - |
Last modified: | 11.04.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 6.0 - 6.5 |
Equipment needed: | Autoclave, Filter, Gassing station, Hungate tubes |
Edit in Medium builder |
Detailed instructions for the cultivation of anaerobes including important warnings can be found in the cultivation instructions .
All strains for this medium: | 1 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
NaCl | 19.96 | 341.548 |
MgSO4 x 7 H2O | 3.443 | 13.969 |
MgCl2 x 6 H2O | 2.745 | 13.5 |
D-Glucose | 1.996 | 11.079 |
Na2CO3 | 0.998 | 9.416 |
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