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1011d. GALENEA MJ MEDIUM (CO2)

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave Hungate tubes
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 30.00 g 29.412 503.26
2 0.14 g 0.137 0.788
3 0.14 g 0.137 0.934
4 3.40 g 3.333 13.524
5 4.18 g 4.098 20.157
6 0.33 g 0.324 4.34
7 0.25 g 0.245 4.582
8 0.01 g 0.01 0.025
9 Trace element solution 10.00 ml - -
10 2.50 g 2.451 23.125
11 1.50 g 1.471 5.925
12 Vitamin solution 10.00 ml - -
13 1000.00 ml 978.634 54322.4
Instructions:
  1. Dissolve ingredients (except carbonate, thiosulfate and vitamins), then sparge medium with 100% CO2 gas for 30 - 45 min to make it anoxic. Dispense medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials up to a volume of 20% and autoclave. Add carbonate, thiosulfate and vitamins to the autoclaved medium from sterile anoxic stock solutions. Solutions of vitamins and thiosulfate are sterilized by filtration and stored under 100% N2 gas, whereas the stock solution of carbonate is prepared under 80% N2 and 20% CO2 gas mixture and autoclaved. Adjust pH of the complete medium to 6.7. After inoculation pressurize vessels to 0.5 bar overpressure with sterile 100% CO2 gas and add sterile air in an amount that is equivalent to a volume of 20% of the headspace.
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 6.7
Equipment needed: Autoclave, Filter, Hungate tubes

Associated strains

All strains for this medium: 1
Strains with modifications: 1

Gas composition

  • 100 % CO2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 29.422 503.428
MgCl2 x 6 H2O 4.098 20.157
MgSO4 x 7 H2O 3.363 13.643
Na2CO3 2.451 23.125
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Cultivation metadata from 1 strains BacDive

halophily not 1 strain
halophily slightly 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
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