1012
1012 .
PEREDIBACTER (DN) MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1012 #
ml
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nutrient broth
(BD Difco) |
0.8 | g | 0.8 | - | |
|
Casamino acids
(BD Bacto) |
0.5 | g | 0.5 | - | |
|
Yeast extract
(OXOID) |
0.1 | g | 0.1 | - | |
| CaCl2 x 2 H2O | 0.3 | g | 0.3 | 2.041 | |
| MgCl2 x 6 H2O | 0.6 | g | 0.6 | 2.951 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Dissolve ingredients except calcium and magnesium chloride and adjust pH of the medium to 7.2 with NaOH. After autoclaving add calcium and magnesium from sterile stock solutions. | |||||
| 2 Before inoculation prepare a suspension of prey cells in 30-40 ml medium using a 250 ml flask. Usually the resuspension of two overnight cultures of the prey strain on agar slants of DSMZ medium 54 will yield a final concentration of 108 to 109 cells per ml culture broth. | |||||
Metadata
| Last modified: | 17.10.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |
Associated strains
| All strains for this medium: | 4 |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| Nutrient broth | 0.8 | - |
| MgCl2 x 6 H2O | 0.6 | 2.951 |
| Casamino acids | 0.5 | - |
| CaCl2 x 2 H2O | 0.3 | 2.041 |
| Yeast extract | 0.1 | - |
| Show more | ||
