1012a
1012a .
BDELLOVIBRIO BACTERIOVORUS MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Main sol. 1012a #
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
Nutrient broth
(BD Difco) |
0.8 | g | 0.8 | - | |
|
Casamino acids
(BD Bacto) |
0.5 | g | 0.5 | - | |
|
Yeast extract
(OXOID) |
0.1 | g | 0.1 | - | |
|
Agar
(BD Bacto) |
12.0 | g | 12 | - | |
| MgCl2 x 6 H2O | 0.6 | g | 0.6 | 2.951 | |
| CaCl2 x 2 H2O | 0.3 | g | 0.3 | 2.041 | |
| Distilled water | 1000.0 | ml | - | - | |
|
1
Preparation of double-layered agar plates: Bottom layer: Dissolve ingredients and adjust the pH of the medium to 7.2 with NaOH. Then bring the medium to a boil to dissolve the agar and autoclave (121°C for 15 min). Dry plates at room temperature for 2 - 3 days. Top layer: Grow prey bacterium on agar slants using DSMZ medium 54 or medium 1. Prepare 5 ml aliquots of 1 % Bacto agar in 25 mM HEPES buffer (6.0 g/l HEPES, 0.6 g/l MgCl2 x 6 H2O, 0.3 g/l CaCl2 x 2 H2O, pH 7.2 ) and autoclave in test tubes. After cooling to 45°C in a water bath add 0.5 ml cell suspension of the host organism, mix, and pour the top layer. For the top layer of one plate use one 24 h old agar slant culture and wash off grown cells in 0.5 ml sterile water.
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| 2 Inoculate the top layer of the double-layer plates the same day when prepared. | |||||
Metadata
| Last modified: | 22.03.24 |
| Source: | MediaDive: DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Petri dishes |
| Edit in Medium builder | |