1012b
1012b .
BACTERIOVORAX MEDIUM
Official DSMZ medium: This medium was manually curated by experts from the DSMZ.
Culture medium recipe
Vol.
×
Main sol. 1012b #
ml
Equipment needed: Autoclave
Petri dishes
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
|
HEPES buffer
(SIGMA H3375) |
6.0 | g | 6 | 25.178 | |
| MgCl2 x 6 H2O | 0.6 | g | 0.6 | 2.951 | |
| CaCl2 x 2 H2O | 0.3 | g | 0.3 | 2.041 | |
| Distilled water | 1000.0 | ml | - | - | |
| 1 Grow prey bacteria on agar plates using the medium indicated below. Use one plate of a 24-hour-old culture and wash off grown cells in 5 ml sterile water. | |||||
| 2 Prepare HEPES buffer, adjust pH to 7.2, and autoclave. After cooling to room temperature add 5 ml cell suspension of the host organism to 50 ml HEPES buffer and mix. The prey medium suspension should contain approx. 107 to 109 cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible. | |||||
Metadata
| Last modified: | 22.03.24 |
| Source: | MediaDive: DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 7.2 |
| Equipment needed: | Autoclave, Petri dishes |
| Edit in Medium builder | |
Molecular composition
| Compound | Conc. [g/L] | Conc. [mM] |
|---|---|---|
| HEPES buffer | 6 | 25.178 |
| MgCl2 x 6 H2O | 0.6 | 2.951 |
| CaCl2 x 2 H2O | 0.3 | 2.041 |
| Distilled water | - | - |
Cultivation metadata from 2 strains
| phyla | 1 | 2 strains |