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1013. HALOBACTERIOVORAX MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 Artificial sea water 700 ml - -
2 Trace element solution 10 ml - -
3 300 ml 296.497 16458.1
Instructions:
  1. Mix ingredients and adjust pH of the final solution to 7.5 - 8.0. Sterilize by autoclaving at 121°C for 20 min. Grow prey bacterium () on agar plates using medium 514. For 50 ml prey medium suspension use one agar plate incubated for 24 hours and wash off grown cells in 3 - 5 ml medium. The prey medium suspension should contain approx. 107 to 109 cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible.
Solution occurs in 3 other media (first defined in medium 343 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 27.70 g 27.7 473.97
2 7.00 g 7 28.4
3 5.50 g 5.5 27.053
4 0.65 g 0.65 8.719
5 0.10 g 0.1 0.972
6 30.00 mg 0.03 0.485
7 15.00 mg 0.015 0.056
8 10.00 mg 0.01 0.052
9 0.05 mg 5.0e-5 1.9e-4
10 2.25 g 2.25 15.305
11 1000.00 ml 998.207 55408.8
Solution occurs in 192 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 1.50 g 1.5 7.848
2 3.00 g 3 12.172
3 0.50 g 0.5 2.958
4 1.00 g 1 17.111
5 0.10 g 0.1 0.36
6 0.18 g 0.18 0.64
7 0.10 g 0.1 0.68
8 0.18 g 0.18 0.626
9 0.01 g 0.01 0.04
10 0.02 g 0.02 0.042
11 0.01 g 0.01 0.162
12 0.01 g 0.01 0.041
13 0.03 g 0.03 0.126
14 0.30 mg 3.0e-4 0.001
15 0.40 mg 4.0e-4 0.001
16 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: unknown

Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.8
NaCl 19.208 328.663
MgSO4 x 7 H2O 4.881 19.804
MgCl2 x 6 H2O 3.812 18.75
CaCl2 x 2 H2O 1.56 10.614
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains

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