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DSMZ

DSMZ 1013 . HALOBACTERIOVORAX MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×

Main sol. 1013 #

ml
Equipment needed: Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Artificial sea water 700 ml - -
Modified Wolin's mineral solution 10 ml - -
Distilled water 300 ml - -
1 Mix ingredients and adjust pH of the final solution to 7.5 - 8.0. Sterilize by autoclaving at 121°C for 20 min. Grow prey bacterium (DSM 15416) on agar plates using medium 514. For 50 ml prey medium suspension use one agar plate incubated for 24 hours and wash off grown cells in 3 - 5 ml medium. The prey medium suspension should contain approx. 107 to 109 cells. Inoculate the complete medium immediately after preparation and incubate the suspension with shaking until a decrease in turbidity is visible.

Artificial sea water #

1000
Solution occurs in 4 other media (first defined in Medium 343).
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaCl 27.70 g 27.7 473.99
MgSO4 x 7 H2O 7.00 g 7 28.4
MgCl2 x 6 H2O 5.50 g 5.5 27.053
KCl 0.65 g 0.65 8.719
NaBr 0.10 g 0.1 0.972
H3BO3 30.00 mg 0.03 0.485
SrCl2 x 6 H2O 15.00 mg 0.015 0.056
Citric acid 10.00 mg 0.01 0.052
KI 0.05 mg 5.0e-5 1.9e-4
CaCl2 x 2 H2O 2.25 g 2.25 15.305
Distilled water 1000.00 ml - -

Modified Wolin's mineral solution #

1000
Solution occurs in 166 other media (first defined in Medium 141).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Nitrilotriacetic acid 1.50 g 1.5 7.848
MgSO4 x 7 H2O 3.00 g 3 12.172
MnSO4 x H2O 0.50 g 0.5 2.958
NaCl 1.00 g 1 17.112
FeSO4 x 7 H2O 0.10 g 0.1 0.36
CoSO4 x 7 H2O 0.18 g 0.18 1.161
CaCl2 x 2 H2O 0.10 g 0.1 0.68
ZnSO4 x 7 H2O 0.18 g 0.18 0.626
CuSO4 x 5 H2O 0.01 g 0.01 0.04
AlK(SO4)2 x 12 H2O 0.02 g 0.02 0.042
H3BO3 0.01 g 0.01 0.162
Na2MoO4 x 2 H2O 0.01 g 0.01 0.041
NiCl2 x 6 H2O 0.03 g 0.03 0.231
Na2SeO3 x 5 H2O 0.30 mg 3.0e-4 0.001
Na2WO4 x 2 H2O 0.40 mg 4.0e-4 0.001
Distilled water 1000.00 ml - -
1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.

Medium 514 #

1000
Solution occurs in 4 other media (first defined in Medium 514).
Compound Amount Unit Conc. [g/L] Conc. [mM]
Bacto peptone 5.00 g 5 -
Yeast extract
(Bacto ) 		1.00 g 1 -
Fe(III) citrate 0.10 g 0.1 0.408
NaCl 19.45 g 19.45 332.82
MgCl2
(anhydrous) 		5.90 g 5.9 61.968
Na2SO4 3.24 g 3.24 22.811
CaCl2 1.80 g 1.8 16.219
KCl 0.55 g 0.55 7.378
NaHCO3 0.16 g 0.16 1.905
KBr 0.08 g 0.08 0.672
SrCl2 34.00 mg 0.034 0.214
H3BO3 22.00 mg 0.022 0.356
Na-silicate 4.00 mg 0.004 0.033
NaF 2.40 mg 0.002 0.057
(NH4)NO3 1.60 mg 0.002 0.02
Na2HPO4 8.00 mg 0.008 0.056
Distilled water 1000.00 ml - -
1 Final pH should be 7.6 ± 0.2 at 25°C. If using the complete medium from Difco add 37.40 g to 1 litre water.

Metadata

Last modified: 12.04.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Defined medium
Final pH: 7.5 - 8.0
Equipment needed: Autoclave
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
MgCl2 x 6 H2O 3.812 18.75
KCl 0.45 6.043
NaBr 0.069 0.674
MgSO4 x 7 H2O 0.03 0.121
Nitrilotriacetic acid 0.015 0.078
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Cultivation metadata from 3 strains BacDive

phyla 1 3 strains
temperature mesophilic 3 strains