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1019. ROUF'S MEDIUM

Culture medium recipe

Vol.
×
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 5.000 g 4.946 -
2 5.000 g 4.946 -
3 0.200 g 0.198 0.803
4 0.050 g 0.049 0.226
5 0.015 g 0.015 -
6 0.050 g 0.049 0.222
7 0.010 g 0.01 0.042
8 Vitamin solution 10.000 ml - -
9 Trace elements solution 1.000 ml - -
10 1000.000 ml 987.346 54806
12 , might need strain adjustment 17.000 g/l - -
Instructions:
  1. pH 7.1 (adjusted with NaOH if needed)
  2. The medium may be solifidied with 17 g/l agar.
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 12.80 g 12.8 66.967
2 0.17 g 0.17 0.714
3 0.10 g 0.1 0.68
4 0.10 g 0.1 0.36
5 0.10 g 0.1 0.505
6 0.10 g 0.1 1.711
7 0.10 g 0.1 0.413
8 1000.00 ml 998.207 55408.8
Instructions:
  1. First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH.
Solution occurs in 366 other media (first defined in medium 141 )
# Compound Amount Unit Conc. [g/L] Conc. [mM]
1 2.0 mg 0.002 0.008
2 2.0 mg 0.002 0.005
3 10.0 mg 0.01 0.049
4 5.0 mg 0.005 0.015
5 5.0 mg 0.005 0.013
6 5.0 mg 0.005 0.041
7 5.0 mg 0.005 0.01
8 0.1 mg 1.0e-4 6.3e-5
9 5.0 mg 0.005 0.036
10 5.0 mg 0.005 0.024
11 1000.0 ml 998.207 55408.8

Metadata

Media status: Official DSMZ medium
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 7.1

Associated strains

All strains for this medium: 2

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Distilled water 998.207 55408.9
Peptone 4.946 -
Yeast extract 4.946 -
MgSO4 x 7 H2O 0.198 0.803
CaCl2 x 6 H2O 0.049 0.226
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Cultivation metadata from 2 strains BacDive

halophily not 1 strain
pH 4 - 6 1 strain
pH 6 - 8 1 strain
pH 8 - 10 1 strain
phyla 1 2 strains
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