This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 5.000 | g | 5 | - | |
Peptone | 5.000 | g | 5 | - | |
MgSO4 x 7 H2O | 0.200 | g | 0.2 | 0.811 | |
CaCl2 x 6 H2O | 0.050 | g | 0.05 | 0.228 | |
Fe(NH4)citrate | 0.015 | g | 0.015 | - | |
MnSO4 x 4 H2O | 0.050 | g | 0.05 | 0.224 | |
FeCl3 x 4 H2O | 0.010 | g | 0.01 | 0.062 | |
Wolin's vitamin solution | 10.000 | ml | - | - | |
Trace elements solution | 1.000 | ml | - | - | |
Distilled water | 1000.000 | ml | - | - | |
Agar , for solid medium | 17.000 | g | 17 | 50.546 | |
1 pH 7.1 (adjusted with NaOH if needed) | |||||
2 The medium may be solifidied with 17 g/l agar. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Nitrilotriacetic acid | 12.80 | g | 12.8 | 66.967 | |
CoCl2 x 6 H2O | 0.17 | g | 0.17 | 1.309 | |
CaCl2 x 2 H2O | 0.10 | g | 0.1 | 0.68 | |
FeSO4 x 7 H2O | 0.10 | g | 0.1 | 0.36 | |
MnCl2 x 4 H2O | 0.10 | g | 0.1 | 0.505 | |
NaCl | 0.10 | g | 0.1 | 1.711 | |
Na2MoO4 x 2 H2O | 0.10 | g | 0.1 | 0.413 | |
Distilled water | 1000.00 | ml | - | - | |
1 First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
Biotin | 2.0 | mg | 0.002 | 0.008 |
Folic acid | 2.0 | mg | 0.002 | 0.005 |
Pyridoxine hydrochloride | 10.0 | mg | 0.01 | 0.049 |
Thiamine HCl | 5.0 | mg | 0.005 | 0.015 |
Riboflavin | 5.0 | mg | 0.005 | 0.013 |
Nicotinic acid | 5.0 | mg | 0.005 | 0.041 |
Calcium D-(+)-pantothenate | 5.0 | mg | 0.005 | 0.021 |
Vitamin B12 | 0.1 | mg | 1.0e-4 | 7.4e-5 |
p-Aminobenzoic acid | 5.0 | mg | 0.005 | 0.036 |
(DL)-alpha-Lipoic acid | 5.0 | mg | 0.005 | 0.024 |
Distilled water | 1000.0 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 7.1 |
Edit in Medium builder |
All strains for this medium: | 2 |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 17 | 50.546 |
Yeast extract | 5 | - |
Peptone | 5 | - |
MgSO4 x 7 H2O | 0.2 | 0.811 |
CaCl2 x 6 H2O | 0.05 | 0.228 |
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