1019 .
ROUF'S MEDIUM
What is this page?
Equipment needed:
Final pH:
7.1
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Yeast extract | 5.000 | g | ||
|
|
Peptone | 5.000 | g | ||
|
|
MgSO4 x 7 H2O | 0.200 | g | ||
|
|
CaCl2 x 6 H2O | 0.050 | g | ||
|
|
Fe(NH4)citrate | 0.015 | g | ||
|
|
MnSO4 x 4 H2O | 0.050 | g | ||
|
|
FeCl3 x 4 H2O | 0.010 | g | ||
|
|
Wolin's vitamin solution | 10.000 | ml | ||
|
|
Trace elements solution | 1.000 | ml | ||
|
|
Distilled water | 1000.000 | ml | ||
|
|
Agar , for solid medium | 17.000 | g | ||
| 1 pH 7.1 (adjusted with NaOH if needed) | |||||
| 2 The medium may be solifidied with 17 g/l agar. | |||||
Trace elements solution #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Nitrilotriacetic acid | 12.80 | g | ||
|
|
CoCl2 x 6 H2O | 0.17 | g | ||
|
|
CaCl2 x 2 H2O | 0.10 | g | ||
|
|
FeSO4 x 7 H2O | 0.10 | g | ||
|
|
MnCl2 x 4 H2O | 0.10 | g | ||
|
|
NaCl | 0.10 | g | ||
|
|
Na2MoO4 x 2 H2O | 0.10 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 First dissolve the nitrilotriacetic acid and adjust the pH to 6.5 with KOH, then add the minerals. The final pH is 7.0, adjusted with KOH. | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
|
Biotin | 2.0 | mg |
|
|
Folic acid | 2.0 | mg |
|
|
Pyridoxine hydrochloride | 10.0 | mg |
|
|
Thiamine HCl | 5.0 | mg |
|
|
Riboflavin | 5.0 | mg |
|
|
Nicotinic acid | 5.0 | mg |
|
|
Calcium D-(+)-pantothenate | 5.0 | mg |
|
|
Vitamin B12 | 0.1 | mg |
|
|
p-Aminobenzoic acid | 5.0 | mg |
|
|
(DL)-alpha-Lipoic acid | 5.0 | mg |
|
|
Distilled water | 1000.0 | ml |