This medium was extracted automatically and has not been curated by experts, yet.
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Solution A | 900 | ml | - | - | |
Solution B | 100 | ml | - | - | |
1 Sterilize solution A and B separately at 121°C for 20 min. Cool to 50°C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
---|---|---|---|---|---|
Yeast extract | 6.20 | g | 6.889 | - | |
Lactalbumin hydrolysate OR Casein hydrolysate | 8.10 | g | 9 | - | |
D-Glucose | 5.00 | g | 5.556 | 30.837 | |
KCl | 0.25 | g | 0.278 | 3.726 | |
MgCl2 x 6 H2O | 0.12 | g | 0.133 | 0.656 | |
CaCl2 x 2 H2O | 0.25 | g | 0.278 | 1.889 | |
NaCl | 8.70 | g | 9.667 | 165.412 | |
NaHCO3 | 0.15 | g | 0.167 | 1.984 | |
Agar | 17.00 | g | 18.889 | 56.162 | |
Distilled water | 900.00 | ml | - | - | |
1 Adjust pH to 8.0. |
Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
---|---|---|---|---|
NaH2PO4 x 2 H2O | 0.28 | g | 2.8 | 23.338 |
Distilled water | 100.00 | ml | - | - |
Last modified: | 22.02.22 |
Source: | DSMZ |
Taxonomic range: | Bacteria |
Medium type: | Complex medium |
Final pH: | 8.0 |
Equipment needed: | Autoclave, Filter |
Edit in Medium builder |
Compound | Conc. [g/L] | Conc. [mM] |
---|---|---|
Agar | 17 | 50.546 |
NaCl | 8.7 | 148.871 |
Lactalbumin hydrolysate | 8.1 | - |
Casein hydrolysate | 8.1 | - |
Yeast extract | 6.2 | - |
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