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DSMZ

DSMZ 1021 . SODALIS GLOSSINIDIUS MEDIUM

This medium was extracted automatically and has not been curated by experts, yet.

Culture medium recipe

Vol.
×

Main sol. 1021 #

ml
Compound Amount Unit Conc. [g/L] Conc. [mM]
Solution A 900 ml - -
Solution B 100 ml - -
1 Sterilize solution A and B separately at 121°C for 20 min. Cool to 50°C, then combine. To 4 parts of the above, add 1 part sterile foetal or new borne calf serum. Liquid medium should be filter sterilized instead of autoclaved.

Solution A #

900
Compound Amount Unit Conc. [g/L] Conc. [mM]
Yeast extract 6.20 g 6.889 -
Lactalbumin hydrolysate OR Casein hydrolysate 8.10 g 9 -
D-Glucose 5.00 g 5.556 30.837
KCl 0.25 g 0.278 3.726
MgCl2 x 6 H2O 0.12 g 0.133 0.656
CaCl2 x 2 H2O 0.25 g 0.278 1.889
NaCl 8.70 g 9.667 165.412
NaHCO3 0.15 g 0.167 1.984
Agar 17.00 g 18.889 56.162
Distilled water 900.00 ml - -
1 Adjust pH to 8.0.

Solution B #

100
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
NaH2PO4 x 2 H2O 0.28 g 2.8 23.338
Distilled water 100.00 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: 8.0
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 1
Strains with modifications: 1

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Agar 17 50.546
NaCl 8.7 148.871
Lactalbumin hydrolysate 8.1 -
Casein hydrolysate 8.1 -
Yeast extract 6.2 -
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Cultivation metadata from 1 strains BacDive

phyla 1 1 strain
oxygen microaerophile 1 strain
temperature mesophilic 1 strain