1023
1023 .
MANNING MEDIUM
This medium was extracted automatically and has not been curated by experts, yet.
Culture medium recipe
Vol.
×
Main sol. 1023 #
ml
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] | |
|---|---|---|---|---|---|
| (NH4)2SO4 | 6.00 | g | 6 | 45.406 | |
| KCl | 0.20 | g | 0.2 | 2.683 | |
| K2HPO4 | 0.20 | g | 0.2 | 1.148 | |
| MgSO4 x 7 H2O | 1.00 | g | 1 | 4.057 | |
| Ca(NO3)2 | 0.02 | g | 0.02 | 0.122 | |
| Distilled water | 1000.00 | ml | - | - | |
| 1 Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7. | |||||
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| FeSO4 x 7 H2O | 33.4 | g | 33.4 | 120.137 |
| Distilled water | 1000.0 | ml | - | - |
Equipment needed: Autoclave
| Compound | Amount | Unit | Conc. [g/L] | Conc. [mM] |
|---|---|---|---|---|
| Yeast extract | 0.2 | g | 0.2 | - |
| Distilled water | 1000.0 | ml | - | - |
Metadata
| Last modified: | 01.04.22 |
| Source: | DSMZ |
| Taxonomic range: | Bacteria |
| Medium type: | Complex medium |
| Final pH: | 2.5 - 2.7 |
| Equipment needed: | Autoclave |
| Edit in Medium builder | |