1023 .
MANNING MEDIUM
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Equipment needed:
Autoclave
Final pH:
2.5 - 2.7
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
(NH4)2SO4 | 6.00 | g | ||
|
|
KCl | 0.20 | g | ||
|
|
K2HPO4 | 0.20 | g | ||
|
|
MgSO4 x 7 H2O | 1.00 | g | ||
|
|
Ca(NO3)2 | 0.02 | g | ||
|
|
Distilled water | 1000.00 | ml | ||
| 1 Solution A is separately autoclaved and added to the medium. The medium is supplemented with Solution B (yeast extract). Medium pH adjusted by 0.1 N H2SO4 to pH 2.5 - 2.7. | |||||