1024 .
NITRATIRUPTOR AND NITRATIFRACTOR MEDIUM
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Equipment needed:
Filter
Final pH:
7.0
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
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NaNO3 | 1 | g | ||
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NaHCO3 | 1 | g | ||
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Na2S2O3 x 5 H2O | 1 | g | ||
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Sulfur powder | 3 | g | ||
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Wolin's vitamin solution | 10 | ml | ||
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Modified Wolin's mineral solution II | 10 | ml | ||
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Synthetic seawater | 1000 | ml | ||
| 1 Prepare the medium under an atmosphere of H2/CO2 (80:20) without adding the vitamins and NaHCO3 in serum bottles and seal the serum tubes with butyl rubber stoppers. Steam medium for 3 hours on each of 3 successive days. To the sterile medium add, from filter sterilised stock solutions, the NaHCO3 and vitamin solution. | |||||
| 2 Increase the 80% H2 + 20% CO2 gas phase pressure to 300 kPa. The final pH is 7.0. | |||||
Wolin's vitamin solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
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Biotin | 2.0 | mg |
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Folic acid | 2.0 | mg |
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Pyridoxine hydrochloride | 10.0 | mg |
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Thiamine HCl | 5.0 | mg |
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Riboflavin | 5.0 | mg |
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Nicotinic acid | 5.0 | mg |
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Calcium D-(+)-pantothenate | 5.0 | mg |
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Vitamin B12 | 0.1 | mg |
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p-Aminobenzoic acid | 5.0 | mg |
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(DL)-alpha-Lipoic acid | 5.0 | mg |
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Distilled water | 1000.0 | ml |
Synthetic seawater #
10
| Compound | Amount | Unit | |
|---|---|---|---|
|
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NaCl | 30.00 | g |
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K2HPO4 | 0.14 | g |
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CaCl2 x 2 H2O | 0.14 | g |
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NH4Cl | 0.25 | g |
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MgSO4 x 7 H2O | 3.40 | g |
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MgCl2 x 6 H2O | 4.18 | g |
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KCl | 0.33 | g |
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NiCl2 x 6 H2O | 0.50 | mg |
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Na2SeO3 x 5 H2O | 0.50 | mg |
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Fe(NH4)2(SO4)2 x 6 H2O | 0.01 | g |
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Trace mineral solution | 10.00 | ml |
Trace mineral solution #
1000
| Compound | Amount | Unit | |
|---|---|---|---|
|
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C6H9NO6 | 1.500 | g |
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MgSO4 x 7 H2O | 3.000 | g |
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MnSO4 x 2 H2O | 0.500 | g |
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NaCl | 1.000 | g |
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FeSO4 x 7 H2O | 0.100 | g |
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CoSO4 x 7 H2O | 0.180 | g |
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CaCl2 x 2 H2O | 0.100 | g |
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ZnSO4 x 7 H2O | 0.180 | g |
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CuSO4 x 5 H2O | 0.010 | g |
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AlK(SO4)2 x 12 H2O | 0.020 | g |
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H3BO3 | 0.010 | g |
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Na2MoO4 x 2 H2O | 0.010 | g |
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NiCl2 x 6 H2O | 0.025 | g |
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Na2SeO3 x 5 H2O | 0.300 | mg |
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Distilled water | 1000.000 | ml |
Modified Wolin's mineral solution II #
1000
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
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Nitrilotriacetic acid | 1.500 | g | ||
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MgSO4 x 7 H2O | 3.000 | g | ||
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MnSO4 x H2O | 0.500 | g | ||
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NaCl | 1.000 | g | ||
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FeSO4 x 7 H2O | 0.100 | g | ||
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CoSO4 x 7 H2O | 0.180 | g | ||
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CaCl2 x 2 H2O | 0.100 | g | ||
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ZnSO4 x 7 H2O | 0.180 | g | ||
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CuSO4 x 5 H2O | 0.010 | g | ||
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AlK(SO4)2 x 12 H2O | 0.020 | g | ||
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H3BO3 | 0.010 | g | ||
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Na2MoO4 x 2 H2O | 0.010 | g | ||
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NiCl2 x 6 H2O | 0.025 | g | ||
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Na2SeO3 x 5 H2O | 0.300 | mg | ||
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Distilled water | 1000.000 | ml | ||
| 1 First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH. | |||||