DSMZ 1025 . MARICAULIS MEDIUM

Official DSMZ medium: This medium was manually curated by experts from the DSMZ.

Culture medium recipe

Vol.
×
Equipment needed: Filter Autoclave
Compound Amount Unit Conc. [g/L] Conc. [mM]
Sigma Sea salts 30.0 g 30 -
NH4Cl 0.5 g 0.5 9.348
Distilled water 1000.0 ml - -
50xPYE 20.0 ml - -
Riboflavin
(0.2 mg/ml)
5.0 ml - -
Glucose
(50 %)
2.0 ml 1 5.551
1 Autoclave and cool the medium. Aseptically add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium.

50xPYE #

1000
Compound Amount Unit Conc. [g/L] Conc. [mM]
Peptone 100 g 100 -
Yeast extract 50 g 50 -
Distilled water 1000 ml - -

Metadata

Last modified: 22.02.22
Source: DSMZ
Taxonomic range:  Bacteria
Medium type: Complex medium
Final pH: n.d.
Equipment needed: Autoclave, Filter
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Associated strains

All strains for this medium: 3

Molecular composition

Compound Conc. [g/L] Conc. [mM]
Sigma Sea salts 30 -
Peptone 2 -
Glucose 1 5.551
Yeast extract 1 -
NH4Cl 0.5 9.348
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Cultivation metadata from 3 strains BacDive

phyla 1 4 strains
temperature mesophilic 4 strains