1025 .
MARICAULIS MEDIUM
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Equipment needed:
Autoclave
Filter
Final pH:
n.d.
Select a strain (optional):
ml
| Compound | Amount | Unit | |||
|---|---|---|---|---|---|
|
|
Sigma Sea salts | 30.0 | g | ||
|
|
NH4Cl | 0.5 | g | ||
|
|
Distilled water | 1000.0 | ml | ||
|
|
50xPYE | 20.0 | ml | ||
|
|
Riboflavin
(0.2 mg/ml) |
5.0 | ml | ||
|
|
Glucose
(50 %) |
2.0 | ml | ||
| 1 Autoclave and cool the medium. Aseptically add from sterile stock solutions, 20 ml 50xPYE and 5 ml 0.2 mg/ml riboflavin (filter sterilised), and 2 ml 50% glucose per liter of medium. | |||||